Measuring the Plasmodium falciparum HRP2 protein in blood from artesunate-treated malaria patients predicts post-artesunate delayed hemolysis
Artesunate, the recommended drug for severe malaria, rapidly clears the malaria parasite from infected patients but frequently induces anemia-called post-artesunate delayed hemolysis (PADH)-for which a simple predictive test is urgently needed. The underlying event in PADH is the expulsion of artesu...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Jezik: | English |
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American Association for the Advancement of Science
2017
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| _version_ | 1826280238827438080 |
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| author | Ndour, P Larréché, S Mouri, O Argy, N Gay, F Roussel, C Jauréguiberry, S Perillaud, C Langui, D Biligui, S Chartrel, N Mérens, A Kendjo, E Ghose, A Hassan, M Hossain, M Kingston, H Plewes, K Dondorp, A Danis, M Houzé, S Bonnefoy, S Thellier, M Woodrow, C Buffet, P French Artesunate Working Group |
| author_facet | Ndour, P Larréché, S Mouri, O Argy, N Gay, F Roussel, C Jauréguiberry, S Perillaud, C Langui, D Biligui, S Chartrel, N Mérens, A Kendjo, E Ghose, A Hassan, M Hossain, M Kingston, H Plewes, K Dondorp, A Danis, M Houzé, S Bonnefoy, S Thellier, M Woodrow, C Buffet, P French Artesunate Working Group |
| author_sort | Ndour, P |
| collection | OXFORD |
| description | Artesunate, the recommended drug for severe malaria, rapidly clears the malaria parasite from infected patients but frequently induces anemia-called post-artesunate delayed hemolysis (PADH)-for which a simple predictive test is urgently needed. The underlying event in PADH is the expulsion of artesunate-exposed parasites from their host erythrocytes by pitting. We show that the histidine-rich protein 2 (HRP2) of the malaria parasite Plasmodium falciparum persists in the circulation of artesunate-treated malaria patients in Bangladesh and in French travelers who became infected with malaria in Africa. HRP2 persisted in whole blood (not plasma) of artesunate-treated patients with malaria at higher levels compared to quinine-treated patients. Using an optimized membrane permeabilization method, HRP2 was observed by immunofluorescence, Western blotting, and electron microscopy to persist in once-infected red blood cells from artesunate-treated malaria patients. HRP2 was deposited at the membrane of once-infected red blood cells in a pattern similar to that for ring erythrocyte surface antigen (RESA), a parasite invasion marker. On the basis of these observations, we developed a semiquantitative titration method using a widely available HRP2-based rapid diagnostic dipstick test. Positivity on this test using a 1:500 dilution of whole blood from artesunate-treated patients with malaria collected shortly after parasite clearance predicted subsequent PADH with 89% sensitivity and 73% specificity. These results suggest that adapting an existing HRP2-based rapid diagnostic dipstick test may enable prediction of PADH several days before it occurs in artesunate-treated patients with malaria. |
| first_indexed | 2024-03-07T00:10:42Z |
| format | Journal article |
| id | oxford-uuid:7922f186-0534-4676-a845-be403ad72773 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:10:42Z |
| publishDate | 2017 |
| publisher | American Association for the Advancement of Science |
| record_format | dspace |
| spelling | oxford-uuid:7922f186-0534-4676-a845-be403ad727732022-03-26T20:35:27ZMeasuring the Plasmodium falciparum HRP2 protein in blood from artesunate-treated malaria patients predicts post-artesunate delayed hemolysisJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7922f186-0534-4676-a845-be403ad72773EnglishSymplectic Elements at OxfordAmerican Association for the Advancement of Science2017Ndour, PLarréché, SMouri, OArgy, NGay, FRoussel, CJauréguiberry, SPerillaud, CLangui, DBiligui, SChartrel, NMérens, AKendjo, EGhose, AHassan, MHossain, MKingston, HPlewes, KDondorp, ADanis, MHouzé, SBonnefoy, SThellier, MWoodrow, CBuffet, PFrench Artesunate Working GroupArtesunate, the recommended drug for severe malaria, rapidly clears the malaria parasite from infected patients but frequently induces anemia-called post-artesunate delayed hemolysis (PADH)-for which a simple predictive test is urgently needed. The underlying event in PADH is the expulsion of artesunate-exposed parasites from their host erythrocytes by pitting. We show that the histidine-rich protein 2 (HRP2) of the malaria parasite Plasmodium falciparum persists in the circulation of artesunate-treated malaria patients in Bangladesh and in French travelers who became infected with malaria in Africa. HRP2 persisted in whole blood (not plasma) of artesunate-treated patients with malaria at higher levels compared to quinine-treated patients. Using an optimized membrane permeabilization method, HRP2 was observed by immunofluorescence, Western blotting, and electron microscopy to persist in once-infected red blood cells from artesunate-treated malaria patients. HRP2 was deposited at the membrane of once-infected red blood cells in a pattern similar to that for ring erythrocyte surface antigen (RESA), a parasite invasion marker. On the basis of these observations, we developed a semiquantitative titration method using a widely available HRP2-based rapid diagnostic dipstick test. Positivity on this test using a 1:500 dilution of whole blood from artesunate-treated patients with malaria collected shortly after parasite clearance predicted subsequent PADH with 89% sensitivity and 73% specificity. These results suggest that adapting an existing HRP2-based rapid diagnostic dipstick test may enable prediction of PADH several days before it occurs in artesunate-treated patients with malaria. |
| spellingShingle | Ndour, P Larréché, S Mouri, O Argy, N Gay, F Roussel, C Jauréguiberry, S Perillaud, C Langui, D Biligui, S Chartrel, N Mérens, A Kendjo, E Ghose, A Hassan, M Hossain, M Kingston, H Plewes, K Dondorp, A Danis, M Houzé, S Bonnefoy, S Thellier, M Woodrow, C Buffet, P French Artesunate Working Group Measuring the Plasmodium falciparum HRP2 protein in blood from artesunate-treated malaria patients predicts post-artesunate delayed hemolysis |
| title | Measuring the Plasmodium falciparum HRP2 protein in blood from artesunate-treated malaria patients predicts post-artesunate delayed hemolysis |
| title_full | Measuring the Plasmodium falciparum HRP2 protein in blood from artesunate-treated malaria patients predicts post-artesunate delayed hemolysis |
| title_fullStr | Measuring the Plasmodium falciparum HRP2 protein in blood from artesunate-treated malaria patients predicts post-artesunate delayed hemolysis |
| title_full_unstemmed | Measuring the Plasmodium falciparum HRP2 protein in blood from artesunate-treated malaria patients predicts post-artesunate delayed hemolysis |
| title_short | Measuring the Plasmodium falciparum HRP2 protein in blood from artesunate-treated malaria patients predicts post-artesunate delayed hemolysis |
| title_sort | measuring the plasmodium falciparum hrp2 protein in blood from artesunate treated malaria patients predicts post artesunate delayed hemolysis |
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