Surface labeling of key residues during assembly of the transmembrane pore formed by staphylococcal alpha-hemolysin.
Structural changes in staphylococcal alpha-hemolysin (alpha HL) that occur during oligomerization and pore formation on membranes have been examined by using a simple gel-shift assay to determine the rate of modification of key single-cysteine mutants with the hydrophilic sulfhydryl reagent, 4-aceta...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1994
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