Analysis of epicardial genes in embryonicmouse hearts with flow cytometry
Genetic markers used to define discrete cell populations are seldom expressed exclusively in the population of interest and are, thus, unsuitable when evaluated individually, especially in the absence of spatial and morphological information. Here, we present fluorescence in situ...
| Glavni autori: | , , |
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| Format: | Journal article |
| Jezik: | English |
| Izdano: |
Cell Press
2021
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| Sažetak: | Genetic markers used to define discrete cell populations are seldom expressed exclusively in the population of interest and are, thus, unsuitable when evaluated individually, especially in the absence of spatial and morphological information. Here, we present fluorescence in situ hybridization for flow cytometry to allow simultaneous analysis of multiple marker genes at the single whole-cell level, exemplified by application to the embryonic epicardium. The protocol facilitates
multiplexed quantification of gene and protein expression and temporal changes across specific cell populations. |
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