Analysis of epicardial genes in embryonicmouse hearts with flow cytometry
Genetic markers used to define discrete cell populations are seldom expressed exclusively in the population of interest and are, thus, unsuitable when evaluated individually, especially in the absence of spatial and morphological information. Here, we present fluorescence in situ...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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Cell Press
2021
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| _version_ | 1797077041043996672 |
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| author | Redpath, A Lupu, I-E Smart, N |
| author_facet | Redpath, A Lupu, I-E Smart, N |
| author_sort | Redpath, A |
| collection | OXFORD |
| description | Genetic markers used to define discrete cell populations are seldom expressed exclusively in the population of interest and are, thus, unsuitable when evaluated individually, especially in the absence of spatial and morphological information. Here, we present fluorescence in situ hybridization for flow cytometry to allow simultaneous analysis of multiple marker genes at the single whole-cell level, exemplified by application to the embryonic epicardium. The protocol facilitates
multiplexed quantification of gene and protein expression and temporal changes across specific cell populations. |
| first_indexed | 2024-03-07T00:12:06Z |
| format | Journal article |
| id | oxford-uuid:7996ed24-a98b-41fb-8d3b-6598368e19f4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:12:06Z |
| publishDate | 2021 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:7996ed24-a98b-41fb-8d3b-6598368e19f42022-03-26T20:38:21ZAnalysis of epicardial genes in embryonicmouse hearts with flow cytometryJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7996ed24-a98b-41fb-8d3b-6598368e19f4EnglishSymplectic ElementsCell Press2021Redpath, ALupu, I-ESmart, NGenetic markers used to define discrete cell populations are seldom expressed exclusively in the population of interest and are, thus, unsuitable when evaluated individually, especially in the absence of spatial and morphological information. Here, we present fluorescence in situ hybridization for flow cytometry to allow simultaneous analysis of multiple marker genes at the single whole-cell level, exemplified by application to the embryonic epicardium. The protocol facilitates multiplexed quantification of gene and protein expression and temporal changes across specific cell populations. |
| spellingShingle | Redpath, A Lupu, I-E Smart, N Analysis of epicardial genes in embryonicmouse hearts with flow cytometry |
| title | Analysis of epicardial genes in embryonicmouse hearts with flow cytometry |
| title_full | Analysis of epicardial genes in embryonicmouse hearts with flow cytometry |
| title_fullStr | Analysis of epicardial genes in embryonicmouse hearts with flow cytometry |
| title_full_unstemmed | Analysis of epicardial genes in embryonicmouse hearts with flow cytometry |
| title_short | Analysis of epicardial genes in embryonicmouse hearts with flow cytometry |
| title_sort | analysis of epicardial genes in embryonicmouse hearts with flow cytometry |
| work_keys_str_mv | AT redpatha analysisofepicardialgenesinembryonicmouseheartswithflowcytometry AT lupuie analysisofepicardialgenesinembryonicmouseheartswithflowcytometry AT smartn analysisofepicardialgenesinembryonicmouseheartswithflowcytometry |