A plate-based high-throughput assay for virus stability and vaccine formulation.
Standard methods for assessing the thermal stability of viruses can be time consuming and rather qualitative yet such data is a necessary requisite for vaccine formulation. In this study a novel plate-based thermal scanning assay for virus particle stability has been developed (PaSTRy: Particle Stab...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2012
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| _version_ | 1797077046312042496 |
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| author | Walter, T Ren, J Tuthill, T Rowlands, D Stuart, D Fry, E |
| author_facet | Walter, T Ren, J Tuthill, T Rowlands, D Stuart, D Fry, E |
| author_sort | Walter, T |
| collection | OXFORD |
| description | Standard methods for assessing the thermal stability of viruses can be time consuming and rather qualitative yet such data is a necessary requisite for vaccine formulation. In this study a novel plate-based thermal scanning assay for virus particle stability has been developed (PaSTRy: Particle Stability Thermal Release Assay). Two environment-sensitive fluorescent dyes, with non-overlapping emission spectra and different affinities, are used to accrue simultaneously independent data for the overall stability of the virus capsid, as judged by the exposure of the genome, and for capsid protein stability according to the exposure of hydrophobic side chains which are normally buried. This offers a fast and efficient high-throughput method to optimise vaccine formulation and to investigate the processes of virus uncoating. |
| first_indexed | 2024-03-07T00:12:11Z |
| format | Journal article |
| id | oxford-uuid:799c6869-3cd3-4fb7-8063-42cc8b677e89 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:12:11Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:799c6869-3cd3-4fb7-8063-42cc8b677e892022-03-26T20:38:30ZA plate-based high-throughput assay for virus stability and vaccine formulation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:799c6869-3cd3-4fb7-8063-42cc8b677e89EnglishSymplectic Elements at Oxford2012Walter, TRen, JTuthill, TRowlands, DStuart, DFry, EStandard methods for assessing the thermal stability of viruses can be time consuming and rather qualitative yet such data is a necessary requisite for vaccine formulation. In this study a novel plate-based thermal scanning assay for virus particle stability has been developed (PaSTRy: Particle Stability Thermal Release Assay). Two environment-sensitive fluorescent dyes, with non-overlapping emission spectra and different affinities, are used to accrue simultaneously independent data for the overall stability of the virus capsid, as judged by the exposure of the genome, and for capsid protein stability according to the exposure of hydrophobic side chains which are normally buried. This offers a fast and efficient high-throughput method to optimise vaccine formulation and to investigate the processes of virus uncoating. |
| spellingShingle | Walter, T Ren, J Tuthill, T Rowlands, D Stuart, D Fry, E A plate-based high-throughput assay for virus stability and vaccine formulation. |
| title | A plate-based high-throughput assay for virus stability and vaccine formulation. |
| title_full | A plate-based high-throughput assay for virus stability and vaccine formulation. |
| title_fullStr | A plate-based high-throughput assay for virus stability and vaccine formulation. |
| title_full_unstemmed | A plate-based high-throughput assay for virus stability and vaccine formulation. |
| title_short | A plate-based high-throughput assay for virus stability and vaccine formulation. |
| title_sort | plate based high throughput assay for virus stability and vaccine formulation |
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