Serine-linked PARP1 auto-modification controls PARP inhibitor response.
Poly(ADP-ribose) polymerase 1 (PARP1) and PARP2 are recruited and activated by DNA damage, resulting in ADP-ribosylation at numerous sites, both within PARP1 itself and in other proteins. Several PARP1 and PARP2 inhibitors are currently employed in the clinic or undergoing trials for treatment of va...
| Príomhchruthaitheoirí: | , , , , , , , , , , , , , , , |
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| Formáid: | Journal article |
| Teanga: | English |
| Foilsithe / Cruthaithe: |
Springer Nature
2021
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| _version_ | 1826280437674147840 |
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| author | Prokhorova, E Zobel, F Smith, R Zentout, S Gibbs-Seymour, I Schützenhofer, K Peters, A Groslambert, J Zorzini, V Agnew, T Brognard, J Nielsen, ML Ahel, D Huet, S Suskiewicz, MJ Ahel, I |
| author_facet | Prokhorova, E Zobel, F Smith, R Zentout, S Gibbs-Seymour, I Schützenhofer, K Peters, A Groslambert, J Zorzini, V Agnew, T Brognard, J Nielsen, ML Ahel, D Huet, S Suskiewicz, MJ Ahel, I |
| author_sort | Prokhorova, E |
| collection | OXFORD |
| description | Poly(ADP-ribose) polymerase 1 (PARP1) and PARP2 are recruited and activated by DNA damage, resulting in ADP-ribosylation at numerous sites, both within PARP1 itself and in other proteins. Several PARP1 and PARP2 inhibitors are currently employed in the clinic or undergoing trials for treatment of various cancers. These drugs act primarily by trapping PARP1 on damaged chromatin, which can lead to cell death, especially in cells with DNA repair defects. Although PARP1 trapping is thought to be caused primarily by the catalytic inhibition of PARP-dependent modification, implying that ADP-ribosylation (ADPr) can counteract trapping, it is not known which exact sites are important for this process. Following recent findings that PARP1- or PARP2-mediated modification is predominantly serine-linked, we demonstrate here that serine ADPr plays a vital role in cellular responses to PARP1/PARP2 inhibitors. Specifically, we identify three serine residues within PARP1 (499, 507, and 519) as key sites whose efficient HPF1-dependent modification counters PARP1 trapping and contributes to inhibitor tolerance. Our data implicate genes that encode serine-specific ADPr regulators, HPF1 and ARH3, as potential PARP1/PARP2 inhibitor therapy biomarkers. |
| first_indexed | 2024-03-07T00:13:42Z |
| format | Journal article |
| id | oxford-uuid:7a1f3d8a-9dd3-46d7-9752-42dd1275f13a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:13:42Z |
| publishDate | 2021 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | oxford-uuid:7a1f3d8a-9dd3-46d7-9752-42dd1275f13a2022-03-26T20:41:48ZSerine-linked PARP1 auto-modification controls PARP inhibitor response.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7a1f3d8a-9dd3-46d7-9752-42dd1275f13aEnglishSymplectic ElementsSpringer Nature2021Prokhorova, EZobel, FSmith, RZentout, SGibbs-Seymour, ISchützenhofer, KPeters, AGroslambert, JZorzini, VAgnew, TBrognard, JNielsen, MLAhel, DHuet, SSuskiewicz, MJAhel, IPoly(ADP-ribose) polymerase 1 (PARP1) and PARP2 are recruited and activated by DNA damage, resulting in ADP-ribosylation at numerous sites, both within PARP1 itself and in other proteins. Several PARP1 and PARP2 inhibitors are currently employed in the clinic or undergoing trials for treatment of various cancers. These drugs act primarily by trapping PARP1 on damaged chromatin, which can lead to cell death, especially in cells with DNA repair defects. Although PARP1 trapping is thought to be caused primarily by the catalytic inhibition of PARP-dependent modification, implying that ADP-ribosylation (ADPr) can counteract trapping, it is not known which exact sites are important for this process. Following recent findings that PARP1- or PARP2-mediated modification is predominantly serine-linked, we demonstrate here that serine ADPr plays a vital role in cellular responses to PARP1/PARP2 inhibitors. Specifically, we identify three serine residues within PARP1 (499, 507, and 519) as key sites whose efficient HPF1-dependent modification counters PARP1 trapping and contributes to inhibitor tolerance. Our data implicate genes that encode serine-specific ADPr regulators, HPF1 and ARH3, as potential PARP1/PARP2 inhibitor therapy biomarkers. |
| spellingShingle | Prokhorova, E Zobel, F Smith, R Zentout, S Gibbs-Seymour, I Schützenhofer, K Peters, A Groslambert, J Zorzini, V Agnew, T Brognard, J Nielsen, ML Ahel, D Huet, S Suskiewicz, MJ Ahel, I Serine-linked PARP1 auto-modification controls PARP inhibitor response. |
| title | Serine-linked PARP1 auto-modification controls PARP inhibitor response. |
| title_full | Serine-linked PARP1 auto-modification controls PARP inhibitor response. |
| title_fullStr | Serine-linked PARP1 auto-modification controls PARP inhibitor response. |
| title_full_unstemmed | Serine-linked PARP1 auto-modification controls PARP inhibitor response. |
| title_short | Serine-linked PARP1 auto-modification controls PARP inhibitor response. |
| title_sort | serine linked parp1 auto modification controls parp inhibitor response |
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