Controlling transmembrane ion transport via photo-regulated carrier mobility
Stimuli-responsive transmembrane ion carriers allow for targeted and controllable transport activity, with potential applications as therapeutics for channelopathies and cancer, and in fundamental studies into ion transport phenomena. These applications require OFF–ON activation from a fully inactiv...
| Những tác giả chính: | , |
|---|---|
| Định dạng: | Journal article |
| Ngôn ngữ: | English |
| Được phát hành: |
Royal Society of Chemistry
2022
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| _version_ | 1826309289844670464 |
|---|---|
| author | Bickerton, L Langton, MJ |
| author_facet | Bickerton, L Langton, MJ |
| author_sort | Bickerton, L |
| collection | OXFORD |
| description | Stimuli-responsive transmembrane ion carriers allow for targeted and controllable transport activity, with potential applications as therapeutics for channelopathies and cancer, and in fundamental studies into ion transport phenomena. These applications require OFF–ON activation from a fully inactive state which does not exhibit background activity, but this remains challenging to achieve with synthetic transport systems. Here we introduce a novel mechanism for photo-gating mobile ion carriers, which involves modulating the mobility of the carriers within the lipid bilayer membrane. By appending a membrane-targeting anchor to the carrier using a photo-cleavable linker, the carrier's ion transport activity is fully switched off by suppressing its ability to shuttle between the two aqueous-membrane interfaces of the bilayer. The system can be reactivated rapidly in situ within the membrane by photo-triggered cleavage of the anchor to release the mobile ion carrier. This approach does not involve direct functionalization of the ion binding site of the carrier, and so does not require the de novo design of novel ion binding motifs to implement the photo-caging of activity. This work demonstrates that controlling the mobility of artificial transport systems enables precise control over activity, opening up new avenues for spatio-temporally targeted ionophores. |
| first_indexed | 2024-03-07T07:33:27Z |
| format | Journal article |
| id | oxford-uuid:7ac953b1-6b0c-4e6b-8959-0dbeb6f4a5d3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:33:27Z |
| publishDate | 2022 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | oxford-uuid:7ac953b1-6b0c-4e6b-8959-0dbeb6f4a5d32023-02-10T12:16:10ZControlling transmembrane ion transport via photo-regulated carrier mobilityJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7ac953b1-6b0c-4e6b-8959-0dbeb6f4a5d3EnglishSymplectic ElementsRoyal Society of Chemistry2022Bickerton, LLangton, MJStimuli-responsive transmembrane ion carriers allow for targeted and controllable transport activity, with potential applications as therapeutics for channelopathies and cancer, and in fundamental studies into ion transport phenomena. These applications require OFF–ON activation from a fully inactive state which does not exhibit background activity, but this remains challenging to achieve with synthetic transport systems. Here we introduce a novel mechanism for photo-gating mobile ion carriers, which involves modulating the mobility of the carriers within the lipid bilayer membrane. By appending a membrane-targeting anchor to the carrier using a photo-cleavable linker, the carrier's ion transport activity is fully switched off by suppressing its ability to shuttle between the two aqueous-membrane interfaces of the bilayer. The system can be reactivated rapidly in situ within the membrane by photo-triggered cleavage of the anchor to release the mobile ion carrier. This approach does not involve direct functionalization of the ion binding site of the carrier, and so does not require the de novo design of novel ion binding motifs to implement the photo-caging of activity. This work demonstrates that controlling the mobility of artificial transport systems enables precise control over activity, opening up new avenues for spatio-temporally targeted ionophores. |
| spellingShingle | Bickerton, L Langton, MJ Controlling transmembrane ion transport via photo-regulated carrier mobility |
| title | Controlling transmembrane ion transport via photo-regulated carrier mobility |
| title_full | Controlling transmembrane ion transport via photo-regulated carrier mobility |
| title_fullStr | Controlling transmembrane ion transport via photo-regulated carrier mobility |
| title_full_unstemmed | Controlling transmembrane ion transport via photo-regulated carrier mobility |
| title_short | Controlling transmembrane ion transport via photo-regulated carrier mobility |
| title_sort | controlling transmembrane ion transport via photo regulated carrier mobility |
| work_keys_str_mv | AT bickertonl controllingtransmembraneiontransportviaphotoregulatedcarriermobility AT langtonmj controllingtransmembraneiontransportviaphotoregulatedcarriermobility |