Production and crystallization of MHC class I B allele single peptide complexes.

Major histocompatibility complex class I B alleles, HLA B8, B53 and B3501 have been cloned, expressed, refolded and crystallized in specific complexes with a number of different 8-mer and 9-mer peptides. For some of these crystallization was initiated by cross-seeding between different B allele comp...

وصف كامل

التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: Reid, S, Smith, K, Jakobsen, B, O'Callaghan, C, Reyburn, H, Harlos, K, Stuart, D, McMichael, A, Bell, J, Jones, E
التنسيق: Journal article
اللغة:English
منشور في: 1996
الوصف
الملخص:Major histocompatibility complex class I B alleles, HLA B8, B53 and B3501 have been cloned, expressed, refolded and crystallized in specific complexes with a number of different 8-mer and 9-mer peptides. For some of these crystallization was initiated by cross-seeding between different B allele complexes. All crystallize in the space group P212121, with similar unit cell dimensions of approximately 52 A X 81 A X 112 A, contain one complex per asymmetric unit and diffract to approximately 2.0 A resolution.