In situ optical mapping of voltage and calcium in the heart.
Electroanatomic mapping the interrelation of intracardiac electrical activation with anatomic locations has become an important tool for clinical assessment of complex arrhythmias. Optical mapping of cardiac electrophysiology combines high spatiotemporal resolution of anatomy and physiological funct...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Public Library of Science
2012
|
| _version_ | 1826280635829846016 |
|---|---|
| author | Lee, P Taghavi, F Yan, P Ewart, P Ashley, E Loew, L Kohl, P Bollensdorff, C Woods, C |
| author_facet | Lee, P Taghavi, F Yan, P Ewart, P Ashley, E Loew, L Kohl, P Bollensdorff, C Woods, C |
| author_sort | Lee, P |
| collection | OXFORD |
| description | Electroanatomic mapping the interrelation of intracardiac electrical activation with anatomic locations has become an important tool for clinical assessment of complex arrhythmias. Optical mapping of cardiac electrophysiology combines high spatiotemporal resolution of anatomy and physiological function with fast and simultaneous data acquisition. If applied to the clinical setting, this could improve both diagnostic potential and therapeutic efficacy of clinical arrhythmia interventions. The aim of this study was to explore this utility in vivo using a rat model. To this aim, we present a single-camera imaging and multiple light-emitting-diode illumination system that reduces economic and technical implementation hurdles to cardiac optical mapping. Combined with a red-shifted calcium dye and a new near-infrared voltage-sensitive dye, both suitable for use in blood-perfused tissue, we demonstrate the feasibility of in vivo multi-parametric imaging of the mammalian heart. Our approach combines recording of electrophysiologically-relevant parameters with observation of structural substrates and is adaptable, in principle, to trans-catheter percutaneous approaches. |
| first_indexed | 2024-03-07T00:16:40Z |
| format | Journal article |
| id | oxford-uuid:7b120044-2db9-46ee-a96a-ee4c53e2cf48 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:16:40Z |
| publishDate | 2012 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:7b120044-2db9-46ee-a96a-ee4c53e2cf482022-03-26T20:48:21ZIn situ optical mapping of voltage and calcium in the heart.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7b120044-2db9-46ee-a96a-ee4c53e2cf48EnglishSymplectic Elements at OxfordPublic Library of Science2012Lee, PTaghavi, FYan, PEwart, PAshley, ELoew, LKohl, PBollensdorff, CWoods, CElectroanatomic mapping the interrelation of intracardiac electrical activation with anatomic locations has become an important tool for clinical assessment of complex arrhythmias. Optical mapping of cardiac electrophysiology combines high spatiotemporal resolution of anatomy and physiological function with fast and simultaneous data acquisition. If applied to the clinical setting, this could improve both diagnostic potential and therapeutic efficacy of clinical arrhythmia interventions. The aim of this study was to explore this utility in vivo using a rat model. To this aim, we present a single-camera imaging and multiple light-emitting-diode illumination system that reduces economic and technical implementation hurdles to cardiac optical mapping. Combined with a red-shifted calcium dye and a new near-infrared voltage-sensitive dye, both suitable for use in blood-perfused tissue, we demonstrate the feasibility of in vivo multi-parametric imaging of the mammalian heart. Our approach combines recording of electrophysiologically-relevant parameters with observation of structural substrates and is adaptable, in principle, to trans-catheter percutaneous approaches. |
| spellingShingle | Lee, P Taghavi, F Yan, P Ewart, P Ashley, E Loew, L Kohl, P Bollensdorff, C Woods, C In situ optical mapping of voltage and calcium in the heart. |
| title | In situ optical mapping of voltage and calcium in the heart. |
| title_full | In situ optical mapping of voltage and calcium in the heart. |
| title_fullStr | In situ optical mapping of voltage and calcium in the heart. |
| title_full_unstemmed | In situ optical mapping of voltage and calcium in the heart. |
| title_short | In situ optical mapping of voltage and calcium in the heart. |
| title_sort | in situ optical mapping of voltage and calcium in the heart |
| work_keys_str_mv | AT leep insituopticalmappingofvoltageandcalciumintheheart AT taghavif insituopticalmappingofvoltageandcalciumintheheart AT yanp insituopticalmappingofvoltageandcalciumintheheart AT ewartp insituopticalmappingofvoltageandcalciumintheheart AT ashleye insituopticalmappingofvoltageandcalciumintheheart AT loewl insituopticalmappingofvoltageandcalciumintheheart AT kohlp insituopticalmappingofvoltageandcalciumintheheart AT bollensdorffc insituopticalmappingofvoltageandcalciumintheheart AT woodsc insituopticalmappingofvoltageandcalciumintheheart |