Multi-compartment encapsulation of communicating droplets and droplet networks in hydrogel as a model for artificial cells.
Constructing a cell mimic is a major challenge posed by synthetic biologists. Efforts to this end have been primarily focused on lipid- and polymer-encapsulated containers, liposomes and polymersomes, respectively. Here, we introduce a multi-compartment, nested system comprising aqueous droplets sta...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Nature Publishing Group
2017
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| _version_ | 1797077371037155328 |
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| author | Bayoumi, M Bayley, J Maglia, G Sapra, K |
| author_facet | Bayoumi, M Bayley, J Maglia, G Sapra, K |
| author_sort | Bayoumi, M |
| collection | OXFORD |
| description | Constructing a cell mimic is a major challenge posed by synthetic biologists. Efforts to this end have been primarily focused on lipid- and polymer-encapsulated containers, liposomes and polymersomes, respectively. Here, we introduce a multi-compartment, nested system comprising aqueous droplets stabilized in an oil/lipid mixture, all encapsulated in hydrogel. Functional capabilities (electrical and chemical communication) were imparted by protein nanopores spanning the lipid bilayer formed at the interface of the encapsulated aqueous droplets and the encasing hydrogel. Crucially, the compartmentalization enabled the formation of two adjoining lipid bilayers in a controlled manner, a requirement for the realization of a functional protocell or prototissue. |
| first_indexed | 2024-03-07T00:16:59Z |
| format | Journal article |
| id | oxford-uuid:7b2f4253-a5c7-462e-ba9d-3d337ddc5180 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:16:59Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | oxford-uuid:7b2f4253-a5c7-462e-ba9d-3d337ddc51802022-03-26T20:48:57ZMulti-compartment encapsulation of communicating droplets and droplet networks in hydrogel as a model for artificial cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7b2f4253-a5c7-462e-ba9d-3d337ddc5180EnglishSymplectic Elements at OxfordNature Publishing Group2017Bayoumi, MBayley, JMaglia, GSapra, KConstructing a cell mimic is a major challenge posed by synthetic biologists. Efforts to this end have been primarily focused on lipid- and polymer-encapsulated containers, liposomes and polymersomes, respectively. Here, we introduce a multi-compartment, nested system comprising aqueous droplets stabilized in an oil/lipid mixture, all encapsulated in hydrogel. Functional capabilities (electrical and chemical communication) were imparted by protein nanopores spanning the lipid bilayer formed at the interface of the encapsulated aqueous droplets and the encasing hydrogel. Crucially, the compartmentalization enabled the formation of two adjoining lipid bilayers in a controlled manner, a requirement for the realization of a functional protocell or prototissue. |
| spellingShingle | Bayoumi, M Bayley, J Maglia, G Sapra, K Multi-compartment encapsulation of communicating droplets and droplet networks in hydrogel as a model for artificial cells. |
| title | Multi-compartment encapsulation of communicating droplets and droplet networks in hydrogel as a model for artificial cells. |
| title_full | Multi-compartment encapsulation of communicating droplets and droplet networks in hydrogel as a model for artificial cells. |
| title_fullStr | Multi-compartment encapsulation of communicating droplets and droplet networks in hydrogel as a model for artificial cells. |
| title_full_unstemmed | Multi-compartment encapsulation of communicating droplets and droplet networks in hydrogel as a model for artificial cells. |
| title_short | Multi-compartment encapsulation of communicating droplets and droplet networks in hydrogel as a model for artificial cells. |
| title_sort | multi compartment encapsulation of communicating droplets and droplet networks in hydrogel as a model for artificial cells |
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