Innate signaling regulates cross-priming at the level of DC licensing and not antigen presentation.
Innate stimuli, such as TLR ligands, are known to greatly facilitate cross-priming. Currently it is unclear whether innate stimuli enhance cross-priming at the level of cross-presentation or at the level of T-cell priming. In this study, we addressed this question by measuring cross-presentation as...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2010
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| _version_ | 1797077373839998976 |
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| author | Keller, SA Schwarz, K Manolova, V von Allmen, C Kinzler, MG Bauer, M Muntwiler, S Saudan, P Bachmann, M |
| author_facet | Keller, SA Schwarz, K Manolova, V von Allmen, C Kinzler, MG Bauer, M Muntwiler, S Saudan, P Bachmann, M |
| author_sort | Keller, SA |
| collection | OXFORD |
| description | Innate stimuli, such as TLR ligands, are known to greatly facilitate cross-priming. Currently it is unclear whether innate stimuli enhance cross-priming at the level of cross-presentation or at the level of T-cell priming. In this study, we addressed this question by measuring cross-presentation as well as cross-priming by virus-like particles (VLP) displaying peptide p33 derived of lymphocytic choriomeningitis virus. Innate stimuli were varied by either packaging different TLR ligands into virus-like particles or using mice deficient in two key molecules of TLR-signaling, namely the adaptor molecule MyD88 as well as IFN-alpha/beta receptor. While efficient cross-presentation occurred despite strongly reduced activation of DC in the absence of TLR ligand-mediated signals, T-cell priming was abolished. Thus, innate stimuli regulate cross-priming at the level of DC licensing for T-cell activation and not antigen presentation. |
| first_indexed | 2024-03-07T00:17:01Z |
| format | Journal article |
| id | oxford-uuid:7b32f46b-82a5-41c0-bbd5-d1aca11c79f6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:17:01Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:7b32f46b-82a5-41c0-bbd5-d1aca11c79f62022-03-26T20:49:05ZInnate signaling regulates cross-priming at the level of DC licensing and not antigen presentation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7b32f46b-82a5-41c0-bbd5-d1aca11c79f6EnglishSymplectic Elements at Oxford2010Keller, SASchwarz, KManolova, Vvon Allmen, CKinzler, MGBauer, MMuntwiler, SSaudan, PBachmann, MInnate stimuli, such as TLR ligands, are known to greatly facilitate cross-priming. Currently it is unclear whether innate stimuli enhance cross-priming at the level of cross-presentation or at the level of T-cell priming. In this study, we addressed this question by measuring cross-presentation as well as cross-priming by virus-like particles (VLP) displaying peptide p33 derived of lymphocytic choriomeningitis virus. Innate stimuli were varied by either packaging different TLR ligands into virus-like particles or using mice deficient in two key molecules of TLR-signaling, namely the adaptor molecule MyD88 as well as IFN-alpha/beta receptor. While efficient cross-presentation occurred despite strongly reduced activation of DC in the absence of TLR ligand-mediated signals, T-cell priming was abolished. Thus, innate stimuli regulate cross-priming at the level of DC licensing for T-cell activation and not antigen presentation. |
| spellingShingle | Keller, SA Schwarz, K Manolova, V von Allmen, C Kinzler, MG Bauer, M Muntwiler, S Saudan, P Bachmann, M Innate signaling regulates cross-priming at the level of DC licensing and not antigen presentation. |
| title | Innate signaling regulates cross-priming at the level of DC licensing and not antigen presentation. |
| title_full | Innate signaling regulates cross-priming at the level of DC licensing and not antigen presentation. |
| title_fullStr | Innate signaling regulates cross-priming at the level of DC licensing and not antigen presentation. |
| title_full_unstemmed | Innate signaling regulates cross-priming at the level of DC licensing and not antigen presentation. |
| title_short | Innate signaling regulates cross-priming at the level of DC licensing and not antigen presentation. |
| title_sort | innate signaling regulates cross priming at the level of dc licensing and not antigen presentation |
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