Antiviral effect of N-butyldeoxynojirimycin against bovine viral diarrhea virus correlates with misfolding of E2 envelope proteins and impairment of their association into E1-E2 heterodimers.
The iminosugar N-butyldeoxynojirimycin (NB-DNJ), an endoplasmic reticulum alpha-glucosidase inhibitor, has an antiviral effect against bovine viral diarrhea virus (BVDV). In this report, we investigate the molecular mechanism of this inhibition by studying the folding pathway of BVDV envelope glycop...
| Egile Nagusiak: | , , , , |
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| Formatua: | Journal article |
| Hizkuntza: | English |
| Argitaratua: |
American Society for Microbiology
2001
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| _version_ | 1826280829881417728 |
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| author | Branza-Nichita, N Durantel, D Carrouée-Durantel, S Dwek, R Zitzmann, N |
| author_facet | Branza-Nichita, N Durantel, D Carrouée-Durantel, S Dwek, R Zitzmann, N |
| author_sort | Branza-Nichita, N |
| collection | OXFORD |
| description | The iminosugar N-butyldeoxynojirimycin (NB-DNJ), an endoplasmic reticulum alpha-glucosidase inhibitor, has an antiviral effect against bovine viral diarrhea virus (BVDV). In this report, we investigate the molecular mechanism of this inhibition by studying the folding pathway of BVDV envelope glycoproteins in the presence and absence of NB-DNJ. Our results show that, while the disulfide-dependent folding of E2 glycoprotein occurs rapidly (2.5 min), the folding of E1 occurs slowly (30 min). Both BVDV envelope glycoproteins associate rapidly with calnexin and dissociate with different kinetics. The release of E1 from the interaction with calnexin coincides with the beginning of E1 and E2 association into disulfide-linked heterodimers. In the presence of NB-DNJ, the interaction of E1 and E2 with calnexin is prevented, leading to misfolding of the envelope glycoproteins and inefficient formation of E1-E2 heterodimers. The degree of misfolding and the lack of association of E1 and E2 into disulfide-linked complexes in the presence of NB-DNJ correlate with the dose-dependent antiviral effect observed for this iminosugar. |
| first_indexed | 2024-03-07T00:19:37Z |
| format | Journal article |
| id | oxford-uuid:7c12f9a4-fa6f-46fc-bcce-2803211a8970 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:19:37Z |
| publishDate | 2001 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | oxford-uuid:7c12f9a4-fa6f-46fc-bcce-2803211a89702022-03-26T20:54:44ZAntiviral effect of N-butyldeoxynojirimycin against bovine viral diarrhea virus correlates with misfolding of E2 envelope proteins and impairment of their association into E1-E2 heterodimers.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7c12f9a4-fa6f-46fc-bcce-2803211a8970EnglishSymplectic Elements at OxfordAmerican Society for Microbiology2001Branza-Nichita, NDurantel, DCarrouée-Durantel, SDwek, RZitzmann, NThe iminosugar N-butyldeoxynojirimycin (NB-DNJ), an endoplasmic reticulum alpha-glucosidase inhibitor, has an antiviral effect against bovine viral diarrhea virus (BVDV). In this report, we investigate the molecular mechanism of this inhibition by studying the folding pathway of BVDV envelope glycoproteins in the presence and absence of NB-DNJ. Our results show that, while the disulfide-dependent folding of E2 glycoprotein occurs rapidly (2.5 min), the folding of E1 occurs slowly (30 min). Both BVDV envelope glycoproteins associate rapidly with calnexin and dissociate with different kinetics. The release of E1 from the interaction with calnexin coincides with the beginning of E1 and E2 association into disulfide-linked heterodimers. In the presence of NB-DNJ, the interaction of E1 and E2 with calnexin is prevented, leading to misfolding of the envelope glycoproteins and inefficient formation of E1-E2 heterodimers. The degree of misfolding and the lack of association of E1 and E2 into disulfide-linked complexes in the presence of NB-DNJ correlate with the dose-dependent antiviral effect observed for this iminosugar. |
| spellingShingle | Branza-Nichita, N Durantel, D Carrouée-Durantel, S Dwek, R Zitzmann, N Antiviral effect of N-butyldeoxynojirimycin against bovine viral diarrhea virus correlates with misfolding of E2 envelope proteins and impairment of their association into E1-E2 heterodimers. |
| title | Antiviral effect of N-butyldeoxynojirimycin against bovine viral diarrhea virus correlates with misfolding of E2 envelope proteins and impairment of their association into E1-E2 heterodimers. |
| title_full | Antiviral effect of N-butyldeoxynojirimycin against bovine viral diarrhea virus correlates with misfolding of E2 envelope proteins and impairment of their association into E1-E2 heterodimers. |
| title_fullStr | Antiviral effect of N-butyldeoxynojirimycin against bovine viral diarrhea virus correlates with misfolding of E2 envelope proteins and impairment of their association into E1-E2 heterodimers. |
| title_full_unstemmed | Antiviral effect of N-butyldeoxynojirimycin against bovine viral diarrhea virus correlates with misfolding of E2 envelope proteins and impairment of their association into E1-E2 heterodimers. |
| title_short | Antiviral effect of N-butyldeoxynojirimycin against bovine viral diarrhea virus correlates with misfolding of E2 envelope proteins and impairment of their association into E1-E2 heterodimers. |
| title_sort | antiviral effect of n butyldeoxynojirimycin against bovine viral diarrhea virus correlates with misfolding of e2 envelope proteins and impairment of their association into e1 e2 heterodimers |
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