Characterization of nuclear structures containing superhelical DNA.
Structures resembling nuclei but depleted of protein may be released by gently lysing cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids sediment in gradients containing intercalating agents in a manner characteristic of DNA that is intact, supercoile...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1976
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