A blueprint for the implementation of a validated approach for the detection of SARS-Cov2 in clinical samples in academic facilities
The COVID-19 pandemic is expanding at an unprecedented rate. As a result, diagnostic services are stretched to their limit, and there is a clear need for the provision of additional diagnostic capacity. Academic laboratories, many of which are closed due to governmental lockdowns, may be in a positi...
| Glavni autori: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Jezik: | English |
| Izdano: |
F1000Research
2020
|
| _version_ | 1826281056968376320 |
|---|---|
| author | Sridhar, S Forrest, S Kean, I Young, J Bartholdson Scott, J Maes, M Pereira-Dias, J Parmar, S Routledge, M Sparkes, D Rivett, L Dougan, G Weekes, M Curran, M Goodfellow, I Baker, S |
| author_facet | Sridhar, S Forrest, S Kean, I Young, J Bartholdson Scott, J Maes, M Pereira-Dias, J Parmar, S Routledge, M Sparkes, D Rivett, L Dougan, G Weekes, M Curran, M Goodfellow, I Baker, S |
| author_sort | Sridhar, S |
| collection | OXFORD |
| description | The COVID-19 pandemic is expanding at an unprecedented rate. As a result, diagnostic services are stretched to their limit, and there is a clear need for the provision of additional diagnostic capacity. Academic laboratories, many of which are closed due to governmental lockdowns, may be in a position to support local screening capacity by adapting their current laboratory practices. Here, we describe the process of developing a SARS-Cov2 diagnostic workflow in a conventional academic Containment Level 2 laboratory. Our outline includes simple SARS-Cov2 deactivation upon contact, the method for a quantitative real-time reverse transcriptase PCR detecting SARS-Cov2, a description of process establishment and validation, and some considerations for establishing a similar workflow elsewhere. This was achieved under challenging circumstances through the collaborative efforts of scientists, clinical staff, and diagnostic staff to mitigate to the ongoing crisis. Within 14 days, we created a validated COVID-19 diagnostics service for healthcare workers in our local hospital. The described methods are not exhaustive, but we hope may offer support to other academic groups aiming to set up something comparable in a short time frame. |
| first_indexed | 2024-03-07T00:23:03Z |
| format | Journal article |
| id | oxford-uuid:7d32d173-ec5b-4e97-9b7f-c559f8c81d16 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:23:03Z |
| publishDate | 2020 |
| publisher | F1000Research |
| record_format | dspace |
| spelling | oxford-uuid:7d32d173-ec5b-4e97-9b7f-c559f8c81d162022-03-26T21:02:03ZA blueprint for the implementation of a validated approach for the detection of SARS-Cov2 in clinical samples in academic facilitiesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7d32d173-ec5b-4e97-9b7f-c559f8c81d16EnglishSymplectic Elements F1000Research2020Sridhar, SForrest, SKean, IYoung, JBartholdson Scott, JMaes, MPereira-Dias, JParmar, SRoutledge, MSparkes, DRivett, LDougan, GWeekes, MCurran, MGoodfellow, IBaker, SThe COVID-19 pandemic is expanding at an unprecedented rate. As a result, diagnostic services are stretched to their limit, and there is a clear need for the provision of additional diagnostic capacity. Academic laboratories, many of which are closed due to governmental lockdowns, may be in a position to support local screening capacity by adapting their current laboratory practices. Here, we describe the process of developing a SARS-Cov2 diagnostic workflow in a conventional academic Containment Level 2 laboratory. Our outline includes simple SARS-Cov2 deactivation upon contact, the method for a quantitative real-time reverse transcriptase PCR detecting SARS-Cov2, a description of process establishment and validation, and some considerations for establishing a similar workflow elsewhere. This was achieved under challenging circumstances through the collaborative efforts of scientists, clinical staff, and diagnostic staff to mitigate to the ongoing crisis. Within 14 days, we created a validated COVID-19 diagnostics service for healthcare workers in our local hospital. The described methods are not exhaustive, but we hope may offer support to other academic groups aiming to set up something comparable in a short time frame. |
| spellingShingle | Sridhar, S Forrest, S Kean, I Young, J Bartholdson Scott, J Maes, M Pereira-Dias, J Parmar, S Routledge, M Sparkes, D Rivett, L Dougan, G Weekes, M Curran, M Goodfellow, I Baker, S A blueprint for the implementation of a validated approach for the detection of SARS-Cov2 in clinical samples in academic facilities |
| title | A blueprint for the implementation of a validated approach for the detection of SARS-Cov2 in clinical samples in academic facilities |
| title_full | A blueprint for the implementation of a validated approach for the detection of SARS-Cov2 in clinical samples in academic facilities |
| title_fullStr | A blueprint for the implementation of a validated approach for the detection of SARS-Cov2 in clinical samples in academic facilities |
| title_full_unstemmed | A blueprint for the implementation of a validated approach for the detection of SARS-Cov2 in clinical samples in academic facilities |
| title_short | A blueprint for the implementation of a validated approach for the detection of SARS-Cov2 in clinical samples in academic facilities |
| title_sort | blueprint for the implementation of a validated approach for the detection of sars cov2 in clinical samples in academic facilities |
| work_keys_str_mv | AT sridhars ablueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT forrests ablueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT keani ablueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT youngj ablueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT bartholdsonscottj ablueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT maesm ablueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT pereiradiasj ablueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT parmars ablueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT routledgem ablueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT sparkesd ablueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT rivettl ablueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT dougang ablueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT weekesm ablueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT curranm ablueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT goodfellowi ablueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT bakers ablueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT sridhars blueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT forrests blueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT keani blueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT youngj blueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT bartholdsonscottj blueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT maesm blueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT pereiradiasj blueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT parmars blueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT routledgem blueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT sparkesd blueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT rivettl blueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT dougang blueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT weekesm blueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT curranm blueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT goodfellowi blueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities AT bakers blueprintfortheimplementationofavalidatedapproachforthedetectionofsarscov2inclinicalsamplesinacademicfacilities |