Methods for quantitative susceptibility and R2* mapping in whole post-mortem brains at 7T applied to amyotrophic lateral sclerosis
Susceptibility weighted magnetic resonance imaging (MRI) is sensitive to the local concentration of iron and myelin. Here, we describe a robust image processing pipeline for quantitative susceptibility mapping (QSM) and R2* mapping of fixed post-mortem, whole-brain data. Using this pipeline, we comp...
| প্রধান লেখক: | , , , , , , , , , , , |
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| বিন্যাস: | Journal article |
| ভাষা: | English |
| প্রকাশিত: |
Elsevier
2020
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| _version_ | 1826281097395175424 |
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| author | Wang, C Foxley, S Ansorge, O Bangerter-Christensen, S Chiew, M Leonte, A Menke, RA Mollink, J Pallebage-Gamarallage, M Turner, MR Miller, KL Tendler, BC |
| author_facet | Wang, C Foxley, S Ansorge, O Bangerter-Christensen, S Chiew, M Leonte, A Menke, RA Mollink, J Pallebage-Gamarallage, M Turner, MR Miller, KL Tendler, BC |
| author_sort | Wang, C |
| collection | OXFORD |
| description | Susceptibility weighted magnetic resonance imaging (MRI) is sensitive to the local concentration of iron and myelin. Here, we describe a robust image processing pipeline for quantitative susceptibility mapping (QSM) and R2* mapping of fixed post-mortem, whole-brain data. Using this pipeline, we compare the resulting quantitative maps in brains from patients with amyotrophic lateral sclerosis (ALS) and controls, with validation against iron and myelin histology.
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Twelve post-mortem brains were scanned with a multi-echo gradient echo sequence at 7T, from which susceptibility and R2* maps were generated. Semi-quantitative histological analysis for ferritin (the principal iron storage protein) and myelin proteolipid protein was performed in the primary motor, anterior cingulate and visual cortices.
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Magnetic susceptibility and R2* values in primary motor cortex were higher in ALS compared to control brains. Magnetic susceptibility and R2* showed positive correlations with both myelin and ferritin estimates from histology. Four out of nine ALS brains exhibited clearly visible hyperintense susceptibility and R2* values in the primary motor cortex.
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Our results demonstrate the potential for MRI-histology studies in whole, fixed post-mortem brains to investigate the biophysical source of susceptibility weighted MRI signals in neurodegenerative diseases like ALS. |
| first_indexed | 2024-03-07T00:23:39Z |
| format | Journal article |
| id | oxford-uuid:7d64a639-cda0-40c2-814f-de797c6f0447 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:23:39Z |
| publishDate | 2020 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:7d64a639-cda0-40c2-814f-de797c6f04472022-03-26T21:03:25ZMethods for quantitative susceptibility and R2* mapping in whole post-mortem brains at 7T applied to amyotrophic lateral sclerosisJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7d64a639-cda0-40c2-814f-de797c6f0447EnglishSymplectic ElementsElsevier 2020Wang, CFoxley, SAnsorge, OBangerter-Christensen, SChiew, MLeonte, AMenke, RAMollink, JPallebage-Gamarallage, MTurner, MRMiller, KLTendler, BCSusceptibility weighted magnetic resonance imaging (MRI) is sensitive to the local concentration of iron and myelin. Here, we describe a robust image processing pipeline for quantitative susceptibility mapping (QSM) and R2* mapping of fixed post-mortem, whole-brain data. Using this pipeline, we compare the resulting quantitative maps in brains from patients with amyotrophic lateral sclerosis (ALS) and controls, with validation against iron and myelin histology. <br></br> Twelve post-mortem brains were scanned with a multi-echo gradient echo sequence at 7T, from which susceptibility and R2* maps were generated. Semi-quantitative histological analysis for ferritin (the principal iron storage protein) and myelin proteolipid protein was performed in the primary motor, anterior cingulate and visual cortices. <br></br> Magnetic susceptibility and R2* values in primary motor cortex were higher in ALS compared to control brains. Magnetic susceptibility and R2* showed positive correlations with both myelin and ferritin estimates from histology. Four out of nine ALS brains exhibited clearly visible hyperintense susceptibility and R2* values in the primary motor cortex. <br></br> Our results demonstrate the potential for MRI-histology studies in whole, fixed post-mortem brains to investigate the biophysical source of susceptibility weighted MRI signals in neurodegenerative diseases like ALS. |
| spellingShingle | Wang, C Foxley, S Ansorge, O Bangerter-Christensen, S Chiew, M Leonte, A Menke, RA Mollink, J Pallebage-Gamarallage, M Turner, MR Miller, KL Tendler, BC Methods for quantitative susceptibility and R2* mapping in whole post-mortem brains at 7T applied to amyotrophic lateral sclerosis |
| title | Methods for quantitative susceptibility and R2* mapping in whole post-mortem brains at 7T applied to amyotrophic lateral sclerosis |
| title_full | Methods for quantitative susceptibility and R2* mapping in whole post-mortem brains at 7T applied to amyotrophic lateral sclerosis |
| title_fullStr | Methods for quantitative susceptibility and R2* mapping in whole post-mortem brains at 7T applied to amyotrophic lateral sclerosis |
| title_full_unstemmed | Methods for quantitative susceptibility and R2* mapping in whole post-mortem brains at 7T applied to amyotrophic lateral sclerosis |
| title_short | Methods for quantitative susceptibility and R2* mapping in whole post-mortem brains at 7T applied to amyotrophic lateral sclerosis |
| title_sort | methods for quantitative susceptibility and r2 mapping in whole post mortem brains at 7t applied to amyotrophic lateral sclerosis |
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