Astroglia up-regulate transcription and secretion of 'readthrough' acetylcholinesterase following oxidative stress.
Novel and diverse functions of glial cells are currently the focus of much attention [A. Volterra and J. Meldolesi (2005) Nature Rev. 6, 626-640]. Here we present evidence that rat astroglia release acetylcholinesterase (AChE) as part of their response to hypoxic damage. Exposure of astroglia to ter...
| Main Authors: | , , , , , , , |
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| 格式: | Journal article |
| 語言: | English |
| 出版: |
2006
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| _version_ | 1826281133997817856 |
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| author | Bond, C Patel, P Crouch, L Tetlow, N Day, T Abu-Hayyeh, S Williamson, C Greenfield, S |
| author_facet | Bond, C Patel, P Crouch, L Tetlow, N Day, T Abu-Hayyeh, S Williamson, C Greenfield, S |
| author_sort | Bond, C |
| collection | OXFORD |
| description | Novel and diverse functions of glial cells are currently the focus of much attention [A. Volterra and J. Meldolesi (2005) Nature Rev. 6, 626-640]. Here we present evidence that rat astroglia release acetylcholinesterase (AChE) as part of their response to hypoxic damage. Exposure of astroglia to tert-butyl hydroperoxide, and hence oxidative stress, subsequently leads to a switching in mRNA from the classical membrane-bound T-AChE to a preferential increase in the splice variant for a soluble form, R-AChE, This change in expression is reflected in increased perinuclear and reduced cytoplasmic AChE staining of the insulted glial cells, with a concomitant and marked increase in extracellular secretion that peaks at 1 h post-treatment. An analogous increase in R-AChE, over a similar time scale, occurs in response to psychological stress [D. Kaufer et al. (1998) Nature 93, 373-377], as well as to head injury and stroke [E. Shohami et al. (1999) J. Neurotrauma 6, 365-76]. The data presented here suggest that glial cells may be key chemical intermediaries in such situations and, perhaps more generally in pathological conditions involving oxidative stress, such as neurodegeneration. |
| first_indexed | 2024-03-07T00:24:13Z |
| format | Journal article |
| id | oxford-uuid:7d90d743-a950-4601-98b3-fa2efc394797 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:24:13Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:7d90d743-a950-4601-98b3-fa2efc3947972022-03-26T21:04:33ZAstroglia up-regulate transcription and secretion of 'readthrough' acetylcholinesterase following oxidative stress.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7d90d743-a950-4601-98b3-fa2efc394797EnglishSymplectic Elements at Oxford2006Bond, CPatel, PCrouch, LTetlow, NDay, TAbu-Hayyeh, SWilliamson, CGreenfield, SNovel and diverse functions of glial cells are currently the focus of much attention [A. Volterra and J. Meldolesi (2005) Nature Rev. 6, 626-640]. Here we present evidence that rat astroglia release acetylcholinesterase (AChE) as part of their response to hypoxic damage. Exposure of astroglia to tert-butyl hydroperoxide, and hence oxidative stress, subsequently leads to a switching in mRNA from the classical membrane-bound T-AChE to a preferential increase in the splice variant for a soluble form, R-AChE, This change in expression is reflected in increased perinuclear and reduced cytoplasmic AChE staining of the insulted glial cells, with a concomitant and marked increase in extracellular secretion that peaks at 1 h post-treatment. An analogous increase in R-AChE, over a similar time scale, occurs in response to psychological stress [D. Kaufer et al. (1998) Nature 93, 373-377], as well as to head injury and stroke [E. Shohami et al. (1999) J. Neurotrauma 6, 365-76]. The data presented here suggest that glial cells may be key chemical intermediaries in such situations and, perhaps more generally in pathological conditions involving oxidative stress, such as neurodegeneration. |
| spellingShingle | Bond, C Patel, P Crouch, L Tetlow, N Day, T Abu-Hayyeh, S Williamson, C Greenfield, S Astroglia up-regulate transcription and secretion of 'readthrough' acetylcholinesterase following oxidative stress. |
| title | Astroglia up-regulate transcription and secretion of 'readthrough' acetylcholinesterase following oxidative stress. |
| title_full | Astroglia up-regulate transcription and secretion of 'readthrough' acetylcholinesterase following oxidative stress. |
| title_fullStr | Astroglia up-regulate transcription and secretion of 'readthrough' acetylcholinesterase following oxidative stress. |
| title_full_unstemmed | Astroglia up-regulate transcription and secretion of 'readthrough' acetylcholinesterase following oxidative stress. |
| title_short | Astroglia up-regulate transcription and secretion of 'readthrough' acetylcholinesterase following oxidative stress. |
| title_sort | astroglia up regulate transcription and secretion of readthrough acetylcholinesterase following oxidative stress |
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