Rescue of influenza A virus from recombinant DNA.

Rescue of influenza A virus from recombinant DNA.

We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase protei...

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Bibliografische gegevens
Hoofdauteurs: Fodor, E, Devenish, L, Engelhardt, O, Palese, P, Brownlee, G, García-Sastre, A
Formaat: Journal article
Taal:English
Gepubliceerd in: 1999
Omschrijving
Samenvatting:We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.

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