Rescue of influenza A virus from recombinant DNA.

Rescue of influenza A virus from recombinant DNA.

We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase protei...

Full description

Bibliographic Details
Main Authors: Fodor, E, Devenish, L, Engelhardt, O, Palese, P, Brownlee, G, García-Sastre, A
Format: Journal article
Language:English
Published: 1999
Description
Summary:We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.

Similar Items