Rescue of influenza A virus from recombinant DNA.
We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase protei...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1999
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| _version_ | 1826281155983310848 |
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| author | Fodor, E Devenish, L Engelhardt, O Palese, P Brownlee, G García-Sastre, A |
| author_facet | Fodor, E Devenish, L Engelhardt, O Palese, P Brownlee, G García-Sastre, A |
| author_sort | Fodor, E |
| collection | OXFORD |
| description | We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes. |
| first_indexed | 2024-03-07T00:24:33Z |
| format | Journal article |
| id | oxford-uuid:7db18e72-119f-48cb-a1db-eeaf4328b646 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:24:33Z |
| publishDate | 1999 |
| record_format | dspace |
| spelling | oxford-uuid:7db18e72-119f-48cb-a1db-eeaf4328b6462022-03-26T21:05:12ZRescue of influenza A virus from recombinant DNA.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7db18e72-119f-48cb-a1db-eeaf4328b646EnglishSymplectic Elements at Oxford1999Fodor, EDevenish, LEngelhardt, OPalese, PBrownlee, GGarcía-Sastre, AWe have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes. |
| spellingShingle | Fodor, E Devenish, L Engelhardt, O Palese, P Brownlee, G García-Sastre, A Rescue of influenza A virus from recombinant DNA. |
| title | Rescue of influenza A virus from recombinant DNA. |
| title_full | Rescue of influenza A virus from recombinant DNA. |
| title_fullStr | Rescue of influenza A virus from recombinant DNA. |
| title_full_unstemmed | Rescue of influenza A virus from recombinant DNA. |
| title_short | Rescue of influenza A virus from recombinant DNA. |
| title_sort | rescue of influenza a virus from recombinant dna |
| work_keys_str_mv | AT fodore rescueofinfluenzaavirusfromrecombinantdna AT devenishl rescueofinfluenzaavirusfromrecombinantdna AT engelhardto rescueofinfluenzaavirusfromrecombinantdna AT palesep rescueofinfluenzaavirusfromrecombinantdna AT brownleeg rescueofinfluenzaavirusfromrecombinantdna AT garciasastrea rescueofinfluenzaavirusfromrecombinantdna |