The bHLH transcription factor OsPRI1 activates the Setaria viridis PEPC1 promoter in rice
<ul> <li>Photosynthetic efficiency is reduced by the dual role of Rubisco, which acts either as a carboxylase or as an oxygenase, the latter leading to photorespiration. C<sub>4</sub> photosynthesis evolved as a carbon-concentrating mechanism to reduce photorespiration. To en...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Wiley
2024
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| _version_ | 1824459200253657088 |
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| author | Carvalho, P Gomes, C Gonçalves, I Lourenço, TF Vlad, D Langdale, JA Saibo, NJM |
| author_facet | Carvalho, P Gomes, C Gonçalves, I Lourenço, TF Vlad, D Langdale, JA Saibo, NJM |
| author_sort | Carvalho, P |
| collection | OXFORD |
| description | <ul>
<li>Photosynthetic efficiency is reduced by the dual role of Rubisco, which acts either as a carboxylase or as an oxygenase, the latter leading to photorespiration. C<sub>4</sub> photosynthesis evolved as a carbon-concentrating mechanism to reduce photorespiration. To engineer C<sub>4</sub> into a C<sub>3</sub> plant, it is essential to understand how C<sub>4</sub> genes, such as <i>phosphoenolpyruvate carboxylase (PEPC1)</i>, are regulated to be expressed at high levels and in a cell-specific manner.</li>
<li>Yeast one-hybrid screening was used to show that OsPRI1, a rice bHLH transcription factor involved in iron homeostasis, binds to the <i>Setaria viridis PEPC1</i> promoter. This promoter drives mesophyll-specific gene expression in rice. The role of OsPRI1 <i>in planta</i> was characterized using a rice line harbouring <i>SvPEPC1<sub>pro</sub>::GUS</i>.</li>
<li>We show that OsPRI1 activates the <i>S. viridis PEPC1</i> promoter by binding to an N-box in the proximal promoter, and that GUS activity is highly reduced in <i>SvPEPC1<sub>pro</sub>::GUS</i> lines when <i>OsPRI1</i> is mutated. Cross-species comparisons showed that the SvPRI1 homolog binds to the <i>SvPEPC1</i> promoter but the maize ZmPRI1 does not bind to the <i>ZmPEPC1</i> promoter.</li>
<li>Our results suggest that elements of the iron homeostasis pathway were co-opted to regulate <i>PEPC1</i> gene expression during the evolution of some but not all C<sub>4</sub> species.</li>
</ul> |
| first_indexed | 2024-03-07T08:15:44Z |
| format | Journal article |
| id | oxford-uuid:7dd0d136-2795-40c7-95a7-066ca32dcc48 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2025-02-19T04:38:00Z |
| publishDate | 2024 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:7dd0d136-2795-40c7-95a7-066ca32dcc482025-02-10T09:41:59ZThe bHLH transcription factor OsPRI1 activates the Setaria viridis PEPC1 promoter in riceJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7dd0d136-2795-40c7-95a7-066ca32dcc48EnglishSymplectic ElementsWiley2024Carvalho, PGomes, CGonçalves, ILourenço, TFVlad, DLangdale, JASaibo, NJM<ul> <li>Photosynthetic efficiency is reduced by the dual role of Rubisco, which acts either as a carboxylase or as an oxygenase, the latter leading to photorespiration. C<sub>4</sub> photosynthesis evolved as a carbon-concentrating mechanism to reduce photorespiration. To engineer C<sub>4</sub> into a C<sub>3</sub> plant, it is essential to understand how C<sub>4</sub> genes, such as <i>phosphoenolpyruvate carboxylase (PEPC1)</i>, are regulated to be expressed at high levels and in a cell-specific manner.</li> <li>Yeast one-hybrid screening was used to show that OsPRI1, a rice bHLH transcription factor involved in iron homeostasis, binds to the <i>Setaria viridis PEPC1</i> promoter. This promoter drives mesophyll-specific gene expression in rice. The role of OsPRI1 <i>in planta</i> was characterized using a rice line harbouring <i>SvPEPC1<sub>pro</sub>::GUS</i>.</li> <li>We show that OsPRI1 activates the <i>S. viridis PEPC1</i> promoter by binding to an N-box in the proximal promoter, and that GUS activity is highly reduced in <i>SvPEPC1<sub>pro</sub>::GUS</i> lines when <i>OsPRI1</i> is mutated. Cross-species comparisons showed that the SvPRI1 homolog binds to the <i>SvPEPC1</i> promoter but the maize ZmPRI1 does not bind to the <i>ZmPEPC1</i> promoter.</li> <li>Our results suggest that elements of the iron homeostasis pathway were co-opted to regulate <i>PEPC1</i> gene expression during the evolution of some but not all C<sub>4</sub> species.</li> </ul> |
| spellingShingle | Carvalho, P Gomes, C Gonçalves, I Lourenço, TF Vlad, D Langdale, JA Saibo, NJM The bHLH transcription factor OsPRI1 activates the Setaria viridis PEPC1 promoter in rice |
| title | The bHLH transcription factor OsPRI1 activates the Setaria viridis PEPC1 promoter in rice |
| title_full | The bHLH transcription factor OsPRI1 activates the Setaria viridis PEPC1 promoter in rice |
| title_fullStr | The bHLH transcription factor OsPRI1 activates the Setaria viridis PEPC1 promoter in rice |
| title_full_unstemmed | The bHLH transcription factor OsPRI1 activates the Setaria viridis PEPC1 promoter in rice |
| title_short | The bHLH transcription factor OsPRI1 activates the Setaria viridis PEPC1 promoter in rice |
| title_sort | bhlh transcription factor ospri1 activates the setaria viridis pepc1 promoter in rice |
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