| Shrnutí: | <strong>Background and Objectives<br></strong>
Recent outbreaks of Zika virus (ZIKV) in South and Central America have highlighted significant neurological side effects. Concurrence with the inflammatory neuropathy Guillain-Barré syndrome (GBS) is observed in 1:4000 ZIKV cases. Whether the neurological symptoms of ZIKV infection are immune-mediated is unclear. We employed rodent and human live cellular models to screen for anti-peripheral nerve reactive IgG and IgM autoantibodies in sera of ZIKV patients with and without GBS.
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Methods<br></strong>
In this study, 52 ZIKV-GBS patients were compared with 134 ZIKV-infected patients without GBS, and 91 non-ZIKV controls. Positive sera were taken forward for target identification by immunoprecipitation and mass spectrometry, and candidate antigens validated by ELISA and cell-based assays. Autoantibody reactions against glycolipid antigens were also screened on an array.
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Results<br></strong>
Overall, IgG antibody reactivities to rat Schwann cells (SCs) (6.5%) and myelinated co-cultures (9.6%) were significantly higher, albeit infrequent, in the ZIKV-GBS group compared to all controls. IgM antibody immunoreactivity to dorsal root ganglia (DRG) neurones (32.3%) and SCs (19.4%) was more frequently observed in the ZIKV-GBS group compared to other controls, while IgM reactivity to co-cultures was as common in ZIKV and non-ZIKV sera. Strong axonal-binding ZIKV-GBS serum IgG antibodies from one patient were confirmed to react with neurofascin 155 and 186. Serum from a ZIKV non-GBS patient displayed strong myelin-binding and putative anti-lipid antigen reaction characteristics. There was however no significant association of ZIKV-GBS with any known anti-glycolipid antibodies.
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Conclusion<br></strong>
Autoantibody responses in ZIKV-GBS target heterogeneous peripheral nerve antigens suggesting heterogeneity of the humoral immune response despite a common prodromal infection.
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