Combined immunophenotyping and FISH identifies the involvement of B-cells in 5q- syndrome.
The 5q- syndrome is a distinct subtype of myelodysplastic syndrome (MDS) characterized by refractory anemia, deletion of the long arm of chromosome 5, del(5q), as the sole cytogenetic abnormality, and a low frequency of transformation to acute leukemia. Using combined immunophenotyping and fluoresce...
| Hoofdauteurs: | , , , , , , |
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| Formaat: | Journal article |
| Taal: | English |
| Gepubliceerd in: |
2000
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| _version_ | 1826281203285622784 |
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| author | Jaju, R Jones, M Boultwood, J Kelly, S Mason, D Wainscoat, J Kearney, L |
| author_facet | Jaju, R Jones, M Boultwood, J Kelly, S Mason, D Wainscoat, J Kearney, L |
| author_sort | Jaju, R |
| collection | OXFORD |
| description | The 5q- syndrome is a distinct subtype of myelodysplastic syndrome (MDS) characterized by refractory anemia, deletion of the long arm of chromosome 5, del(5q), as the sole cytogenetic abnormality, and a low frequency of transformation to acute leukemia. Using combined immunophenotyping and fluorescence in situ hybridization (FISH), studies were carried out on bone marrow smears of three 5q- syndrome cases to identify the cell lineages carrying the 5q deletion. In all three cases, the granulocytic, monocytic, and erythroid lineages possessed the del(5q) clonal marker, whereas the T-lymphocytes did not. Interestingly, in one case, cells of B-lymphoid lineage also showed the presence of the del(5q). This is the first report to date showing involvement of an acquired 5q deletion associated with MDS in B-cells. This result suggests that in some cases, MDS arises in a multipotent cell with a capacity to differentiate into both myeloid and lymphoid cells. |
| first_indexed | 2024-03-07T00:25:16Z |
| format | Journal article |
| id | oxford-uuid:7def804f-4ece-437e-af7a-57747bfcccc4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:25:16Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:7def804f-4ece-437e-af7a-57747bfcccc42022-03-26T21:06:53ZCombined immunophenotyping and FISH identifies the involvement of B-cells in 5q- syndrome.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7def804f-4ece-437e-af7a-57747bfcccc4EnglishSymplectic Elements at Oxford2000Jaju, RJones, MBoultwood, JKelly, SMason, DWainscoat, JKearney, LThe 5q- syndrome is a distinct subtype of myelodysplastic syndrome (MDS) characterized by refractory anemia, deletion of the long arm of chromosome 5, del(5q), as the sole cytogenetic abnormality, and a low frequency of transformation to acute leukemia. Using combined immunophenotyping and fluorescence in situ hybridization (FISH), studies were carried out on bone marrow smears of three 5q- syndrome cases to identify the cell lineages carrying the 5q deletion. In all three cases, the granulocytic, monocytic, and erythroid lineages possessed the del(5q) clonal marker, whereas the T-lymphocytes did not. Interestingly, in one case, cells of B-lymphoid lineage also showed the presence of the del(5q). This is the first report to date showing involvement of an acquired 5q deletion associated with MDS in B-cells. This result suggests that in some cases, MDS arises in a multipotent cell with a capacity to differentiate into both myeloid and lymphoid cells. |
| spellingShingle | Jaju, R Jones, M Boultwood, J Kelly, S Mason, D Wainscoat, J Kearney, L Combined immunophenotyping and FISH identifies the involvement of B-cells in 5q- syndrome. |
| title | Combined immunophenotyping and FISH identifies the involvement of B-cells in 5q- syndrome. |
| title_full | Combined immunophenotyping and FISH identifies the involvement of B-cells in 5q- syndrome. |
| title_fullStr | Combined immunophenotyping and FISH identifies the involvement of B-cells in 5q- syndrome. |
| title_full_unstemmed | Combined immunophenotyping and FISH identifies the involvement of B-cells in 5q- syndrome. |
| title_short | Combined immunophenotyping and FISH identifies the involvement of B-cells in 5q- syndrome. |
| title_sort | combined immunophenotyping and fish identifies the involvement of b cells in 5q syndrome |
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