Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo.
DNA damage and its repair can cause both local and global rearrangements of chromatin structure. In each case, the epigenetic information contained within this structure must be maintained. Using the recently developed method for the localized UV irradiation of cells, we analysed responses that occu...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
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2003
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| _version_ | 1797077952044728320 |
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| author | Green, C Almouzni, G |
| author_facet | Green, C Almouzni, G |
| author_sort | Green, C |
| collection | OXFORD |
| description | DNA damage and its repair can cause both local and global rearrangements of chromatin structure. In each case, the epigenetic information contained within this structure must be maintained. Using the recently developed method for the localized UV irradiation of cells, we analysed responses that occur locally to damage sites and global events triggered by local damage recognition. We thus demonstrate that, within a single cell, the recruitment of chromatin assembly factor 1 (CAF-1) to UV-induced DNA damage is a strictly local phenomenon, restricted to damage sites. Concomitantly, proliferating cell nuclear antigen (PCNA) locates to the same sites. This localized recruitment suggests that CAF-1 participates directly in chromatin structural rearrangements that occur in the vicinity of the damage. Use of nucleotide excision repair (NER)-deficient cells shows that the NER pathway--specifically dual incision--is required for recruitment of CAF-1 and PCNA. This in vivo demonstration of the local role of CAF-1, depending directly on NER, supports the hypothesis that CAF-1 ensures the maintenance of epigenetic information by acting locally at repair sites. |
| first_indexed | 2024-03-07T00:25:25Z |
| format | Journal article |
| id | oxford-uuid:7dfab4f7-34b9-47c1-aea4-af7d8c758ab1 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:25:25Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:7dfab4f7-34b9-47c1-aea4-af7d8c758ab12022-03-26T21:07:09ZLocal action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7dfab4f7-34b9-47c1-aea4-af7d8c758ab1EnglishSymplectic Elements at Oxford2003Green, CAlmouzni, GDNA damage and its repair can cause both local and global rearrangements of chromatin structure. In each case, the epigenetic information contained within this structure must be maintained. Using the recently developed method for the localized UV irradiation of cells, we analysed responses that occur locally to damage sites and global events triggered by local damage recognition. We thus demonstrate that, within a single cell, the recruitment of chromatin assembly factor 1 (CAF-1) to UV-induced DNA damage is a strictly local phenomenon, restricted to damage sites. Concomitantly, proliferating cell nuclear antigen (PCNA) locates to the same sites. This localized recruitment suggests that CAF-1 participates directly in chromatin structural rearrangements that occur in the vicinity of the damage. Use of nucleotide excision repair (NER)-deficient cells shows that the NER pathway--specifically dual incision--is required for recruitment of CAF-1 and PCNA. This in vivo demonstration of the local role of CAF-1, depending directly on NER, supports the hypothesis that CAF-1 ensures the maintenance of epigenetic information by acting locally at repair sites. |
| spellingShingle | Green, C Almouzni, G Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo. |
| title | Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo. |
| title_full | Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo. |
| title_fullStr | Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo. |
| title_full_unstemmed | Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo. |
| title_short | Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo. |
| title_sort | local action of the chromatin assembly factor caf 1 at sites of nucleotide excision repair in vivo |
| work_keys_str_mv | AT greenc localactionofthechromatinassemblyfactorcaf1atsitesofnucleotideexcisionrepairinvivo AT almouznig localactionofthechromatinassemblyfactorcaf1atsitesofnucleotideexcisionrepairinvivo |