Generation of LexA enhancer-trap lines in Drosophila by an international scholastic network
Conditional gene regulation in Drosophila through binary expression systems like the LexA-LexAop system provides a superb tool for investigating gene and tissue function. To increase the availability of defined LexA enhancer trap insertions, we present molecular, genetic, and tissue expression studi...
| Auteurs principaux: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Langue: | English |
| Publié: |
Genetics Society of America
2023
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| _version_ | 1826313586599788544 |
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| author | Kim, ES Rajan, A Chang, K Govindarajan, S Gulick, C English, E Rodriguez, B Bloomfield, O Nakada, S Beard, C O’Connor, S Mastroianni, S Downey, E Feigenbaum, M Tolentino, C Pace, A Khan, M Moon, S DiPrima, J Syed, A Lin, F Abukhadra, Y Bacon, I Beckerle, J Baena-Lopez, A |
| author_facet | Kim, ES Rajan, A Chang, K Govindarajan, S Gulick, C English, E Rodriguez, B Bloomfield, O Nakada, S Beard, C O’Connor, S Mastroianni, S Downey, E Feigenbaum, M Tolentino, C Pace, A Khan, M Moon, S DiPrima, J Syed, A Lin, F Abukhadra, Y Bacon, I Beckerle, J Baena-Lopez, A |
| author_sort | Kim, ES |
| collection | OXFORD |
| description | Conditional gene regulation in Drosophila through binary expression systems like the LexA-LexAop system provides a superb tool for investigating gene and tissue function. To increase the availability of defined LexA enhancer trap insertions, we present molecular, genetic, and tissue expression studies of 301 novel Stan-X LexA enhancer traps derived from mobilization of the index SX4 line. This includes insertions into distinct loci on the X, II, and III chromosomes that were not previously associated with enhancer traps or targeted LexA constructs, an insertion into ptc, and seventeen insertions into natural transposons. A subset of enhancer traps was expressed in CNS neurons known to produce and secrete insulin, an essential regulator of growth, development, and metabolism. Fly lines described here were generated and characterized through studies by students and teachers in an international network of genetics classes at public, independent high schools, and universities serving a diversity of students, including those underrepresented in science. Thus, a unique partnership between secondary schools and university-based programs has produced and characterized novel resources in Drosophila, establishing instructional paradigms devoted to unscripted experimental science. |
| first_indexed | 2024-09-25T04:17:20Z |
| format | Journal article |
| id | oxford-uuid:7e018b74-9eae-42b7-a656-a7eeee5de6c4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-09-25T04:17:20Z |
| publishDate | 2023 |
| publisher | Genetics Society of America |
| record_format | dspace |
| spelling | oxford-uuid:7e018b74-9eae-42b7-a656-a7eeee5de6c42024-07-20T15:18:59ZGeneration of LexA enhancer-trap lines in Drosophila by an international scholastic networkJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7e018b74-9eae-42b7-a656-a7eeee5de6c4EnglishJisc Publications RouterGenetics Society of America2023Kim, ESRajan, AChang, KGovindarajan, SGulick, CEnglish, ERodriguez, BBloomfield, ONakada, SBeard, CO’Connor, SMastroianni, SDowney, EFeigenbaum, MTolentino, CPace, AKhan, MMoon, SDiPrima, JSyed, ALin, FAbukhadra, YBacon, IBeckerle, JBaena-Lopez, AConditional gene regulation in Drosophila through binary expression systems like the LexA-LexAop system provides a superb tool for investigating gene and tissue function. To increase the availability of defined LexA enhancer trap insertions, we present molecular, genetic, and tissue expression studies of 301 novel Stan-X LexA enhancer traps derived from mobilization of the index SX4 line. This includes insertions into distinct loci on the X, II, and III chromosomes that were not previously associated with enhancer traps or targeted LexA constructs, an insertion into ptc, and seventeen insertions into natural transposons. A subset of enhancer traps was expressed in CNS neurons known to produce and secrete insulin, an essential regulator of growth, development, and metabolism. Fly lines described here were generated and characterized through studies by students and teachers in an international network of genetics classes at public, independent high schools, and universities serving a diversity of students, including those underrepresented in science. Thus, a unique partnership between secondary schools and university-based programs has produced and characterized novel resources in Drosophila, establishing instructional paradigms devoted to unscripted experimental science. |
| spellingShingle | Kim, ES Rajan, A Chang, K Govindarajan, S Gulick, C English, E Rodriguez, B Bloomfield, O Nakada, S Beard, C O’Connor, S Mastroianni, S Downey, E Feigenbaum, M Tolentino, C Pace, A Khan, M Moon, S DiPrima, J Syed, A Lin, F Abukhadra, Y Bacon, I Beckerle, J Baena-Lopez, A Generation of LexA enhancer-trap lines in Drosophila by an international scholastic network |
| title | Generation of LexA enhancer-trap lines in Drosophila by an international scholastic network |
| title_full | Generation of LexA enhancer-trap lines in Drosophila by an international scholastic network |
| title_fullStr | Generation of LexA enhancer-trap lines in Drosophila by an international scholastic network |
| title_full_unstemmed | Generation of LexA enhancer-trap lines in Drosophila by an international scholastic network |
| title_short | Generation of LexA enhancer-trap lines in Drosophila by an international scholastic network |
| title_sort | generation of lexa enhancer trap lines in drosophila by an international scholastic network |
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