CD169(+) macrophages present lipid antigens to mediate early activation of iNKT cells in lymph nodes.
Invariant natural killer T cells (iNKT cells) are involved in the host defense against microbial infection. Although it is known that iNKT cells recognize glycolipids presented by CD1d, how and where they encounter antigen in vivo remains unclear. Here we used multiphoton microscopy to visualize the...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2010
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| _version_ | 1797078034058051584 |
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| author | Barral, P Polzella, P Bruckbauer, A van Rooijen, N Besra, G Cerundolo, V Batista, F |
| author_facet | Barral, P Polzella, P Bruckbauer, A van Rooijen, N Besra, G Cerundolo, V Batista, F |
| author_sort | Barral, P |
| collection | OXFORD |
| description | Invariant natural killer T cells (iNKT cells) are involved in the host defense against microbial infection. Although it is known that iNKT cells recognize glycolipids presented by CD1d, how and where they encounter antigen in vivo remains unclear. Here we used multiphoton microscopy to visualize the dynamics and activation of iNKT cells in lymph nodes. After antigen administration, iNKT cells became confined in a CD1d-dependent manner in close proximity to subcapsular sinus CD169(+) macrophages. These macrophages retained, internalized and presented lipid antigen and were required for iNKT cell activation, cytokine production and population expansion. Thus, CD169(+) macrophages can act as true antigen-presenting cells controlling early iNKT cell activation and favoring the fast initiation of immune responses. |
| first_indexed | 2024-03-07T00:26:39Z |
| format | Journal article |
| id | oxford-uuid:7e5e3945-fc5b-4ab2-b9de-9e85afa7e19d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:26:39Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:7e5e3945-fc5b-4ab2-b9de-9e85afa7e19d2022-03-26T21:09:41ZCD169(+) macrophages present lipid antigens to mediate early activation of iNKT cells in lymph nodes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7e5e3945-fc5b-4ab2-b9de-9e85afa7e19dEnglishSymplectic Elements at Oxford2010Barral, PPolzella, PBruckbauer, Avan Rooijen, NBesra, GCerundolo, VBatista, FInvariant natural killer T cells (iNKT cells) are involved in the host defense against microbial infection. Although it is known that iNKT cells recognize glycolipids presented by CD1d, how and where they encounter antigen in vivo remains unclear. Here we used multiphoton microscopy to visualize the dynamics and activation of iNKT cells in lymph nodes. After antigen administration, iNKT cells became confined in a CD1d-dependent manner in close proximity to subcapsular sinus CD169(+) macrophages. These macrophages retained, internalized and presented lipid antigen and were required for iNKT cell activation, cytokine production and population expansion. Thus, CD169(+) macrophages can act as true antigen-presenting cells controlling early iNKT cell activation and favoring the fast initiation of immune responses. |
| spellingShingle | Barral, P Polzella, P Bruckbauer, A van Rooijen, N Besra, G Cerundolo, V Batista, F CD169(+) macrophages present lipid antigens to mediate early activation of iNKT cells in lymph nodes. |
| title | CD169(+) macrophages present lipid antigens to mediate early activation of iNKT cells in lymph nodes. |
| title_full | CD169(+) macrophages present lipid antigens to mediate early activation of iNKT cells in lymph nodes. |
| title_fullStr | CD169(+) macrophages present lipid antigens to mediate early activation of iNKT cells in lymph nodes. |
| title_full_unstemmed | CD169(+) macrophages present lipid antigens to mediate early activation of iNKT cells in lymph nodes. |
| title_short | CD169(+) macrophages present lipid antigens to mediate early activation of iNKT cells in lymph nodes. |
| title_sort | cd169 macrophages present lipid antigens to mediate early activation of inkt cells in lymph nodes |
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