Peptide orientation affects selectivity in ion-exchange chromatography.
Here we demonstrate that separation of proteolytic peptides, having the same net charge and one basic residue, is affected by their specific orientation toward the stationary phase in ion-exchange chromatography. In electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) with an anion...
Main Authors: | Alpert, A, Petritis, K, Kangas, L, Smith, R, Mechtler, K, Mitulović, G, Mohammed, S, Heck, A |
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Format: | Journal article |
Language: | English |
Published: |
2010
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