The PTS(Ntr) system globally regulates ATP-dependent transporters in Rhizobium leguminosarum.
Mutation of ptsP encoding EI(Ntr) of the PTS(Ntr) system in Rhizobium leguminosarum strain Rlv3841 caused a pleiotropic phenotype as observed with many bacteria. The mutant formed dry colonies and grew poorly on organic nitrogen or dicarboxylates. Most strikingly the ptsP mutant had low activity o...
| Váldodahkkit: | , , , , , , , |
|---|---|
| Materiálatiipa: | Journal article |
| Giella: | English |
| Almmustuhtton: |
2012
|
| _version_ | 1826281372717678592 |
|---|---|
| author | Prell, J Mulley, G Haufe, F White, J Williams, A Karunakaran, R Downie, J Poole, P |
| author_facet | Prell, J Mulley, G Haufe, F White, J Williams, A Karunakaran, R Downie, J Poole, P |
| author_sort | Prell, J |
| collection | OXFORD |
| description | Mutation of ptsP encoding EI(Ntr) of the PTS(Ntr) system in Rhizobium leguminosarum strain Rlv3841 caused a pleiotropic phenotype as observed with many bacteria. The mutant formed dry colonies and grew poorly on organic nitrogen or dicarboxylates. Most strikingly the ptsP mutant had low activity of a broad range of ATP-dependent ABC transporters. This lack of activation, which occurred post-translationally, may explain many of the pleiotropic effects. In contrast proton-coupled transport systems were not inhibited in a ptsP mutant. Regulation by PtsP also involves two copies of ptsN that code for EIIA(Ntr) , resulting in a phosphorylation cascade. As in Escherichia coli, the Rlv3841 PTS(Ntr) system also regulates K(+) homeostasis by transcriptional activation of the high-affinity ATP-dependent K(+) transporter KdpABC. This involves direct interaction of a two-component sensor regulator pair KdpDE with unphosphorylated EIIA(Ntr) . Critically, ptsP mutants, which cannot phosphorylate PtsN1 or PtsN2, had a fully activated KdpABC transporter. This is the opposite pattern from that observed with ABC transporters which apparently require phosphorylation of PtsN. These results suggest that ATP-dependent transport might be regulated via PTS(Ntr) responding to the cellular energy charge. ABC transport may be inactivated at low energy charge, conserving ATP for essential processes including K(+) homeostasis. |
| first_indexed | 2024-03-07T00:27:49Z |
| format | Journal article |
| id | oxford-uuid:7ebced23-4f2f-4c4f-8ec5-2a2f50064c33 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:27:49Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:7ebced23-4f2f-4c4f-8ec5-2a2f50064c332022-03-26T21:12:01ZThe PTS(Ntr) system globally regulates ATP-dependent transporters in Rhizobium leguminosarum.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7ebced23-4f2f-4c4f-8ec5-2a2f50064c33EnglishSymplectic Elements at Oxford2012Prell, JMulley, GHaufe, FWhite, JWilliams, AKarunakaran, RDownie, JPoole, PMutation of ptsP encoding EI(Ntr) of the PTS(Ntr) system in Rhizobium leguminosarum strain Rlv3841 caused a pleiotropic phenotype as observed with many bacteria. The mutant formed dry colonies and grew poorly on organic nitrogen or dicarboxylates. Most strikingly the ptsP mutant had low activity of a broad range of ATP-dependent ABC transporters. This lack of activation, which occurred post-translationally, may explain many of the pleiotropic effects. In contrast proton-coupled transport systems were not inhibited in a ptsP mutant. Regulation by PtsP also involves two copies of ptsN that code for EIIA(Ntr) , resulting in a phosphorylation cascade. As in Escherichia coli, the Rlv3841 PTS(Ntr) system also regulates K(+) homeostasis by transcriptional activation of the high-affinity ATP-dependent K(+) transporter KdpABC. This involves direct interaction of a two-component sensor regulator pair KdpDE with unphosphorylated EIIA(Ntr) . Critically, ptsP mutants, which cannot phosphorylate PtsN1 or PtsN2, had a fully activated KdpABC transporter. This is the opposite pattern from that observed with ABC transporters which apparently require phosphorylation of PtsN. These results suggest that ATP-dependent transport might be regulated via PTS(Ntr) responding to the cellular energy charge. ABC transport may be inactivated at low energy charge, conserving ATP for essential processes including K(+) homeostasis. |
| spellingShingle | Prell, J Mulley, G Haufe, F White, J Williams, A Karunakaran, R Downie, J Poole, P The PTS(Ntr) system globally regulates ATP-dependent transporters in Rhizobium leguminosarum. |
| title | The PTS(Ntr) system globally regulates ATP-dependent transporters in Rhizobium leguminosarum. |
| title_full | The PTS(Ntr) system globally regulates ATP-dependent transporters in Rhizobium leguminosarum. |
| title_fullStr | The PTS(Ntr) system globally regulates ATP-dependent transporters in Rhizobium leguminosarum. |
| title_full_unstemmed | The PTS(Ntr) system globally regulates ATP-dependent transporters in Rhizobium leguminosarum. |
| title_short | The PTS(Ntr) system globally regulates ATP-dependent transporters in Rhizobium leguminosarum. |
| title_sort | pts ntr system globally regulates atp dependent transporters in rhizobium leguminosarum |
| work_keys_str_mv | AT prellj theptsntrsystemgloballyregulatesatpdependenttransportersinrhizobiumleguminosarum AT mulleyg theptsntrsystemgloballyregulatesatpdependenttransportersinrhizobiumleguminosarum AT haufef theptsntrsystemgloballyregulatesatpdependenttransportersinrhizobiumleguminosarum AT whitej theptsntrsystemgloballyregulatesatpdependenttransportersinrhizobiumleguminosarum AT williamsa theptsntrsystemgloballyregulatesatpdependenttransportersinrhizobiumleguminosarum AT karunakaranr theptsntrsystemgloballyregulatesatpdependenttransportersinrhizobiumleguminosarum AT downiej theptsntrsystemgloballyregulatesatpdependenttransportersinrhizobiumleguminosarum AT poolep theptsntrsystemgloballyregulatesatpdependenttransportersinrhizobiumleguminosarum AT prellj ptsntrsystemgloballyregulatesatpdependenttransportersinrhizobiumleguminosarum AT mulleyg ptsntrsystemgloballyregulatesatpdependenttransportersinrhizobiumleguminosarum AT haufef ptsntrsystemgloballyregulatesatpdependenttransportersinrhizobiumleguminosarum AT whitej ptsntrsystemgloballyregulatesatpdependenttransportersinrhizobiumleguminosarum AT williamsa ptsntrsystemgloballyregulatesatpdependenttransportersinrhizobiumleguminosarum AT karunakaranr ptsntrsystemgloballyregulatesatpdependenttransportersinrhizobiumleguminosarum AT downiej ptsntrsystemgloballyregulatesatpdependenttransportersinrhizobiumleguminosarum AT poolep ptsntrsystemgloballyregulatesatpdependenttransportersinrhizobiumleguminosarum |