Scattering-based light microscopy: From metal nanoparticles to single proteins
Our ability to detect, image, and quantify nanoscopic objects and molecules with visible light has undergone dramatic improvements over the past few decades. While fluorescence has historically been the go-to contrast mechanism for ultrasensitive light microscopy due to its superior background suppr...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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American Chemical Society
2021
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| _version_ | 1826281411481436160 |
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| author | Priest, L Peters, JS Kukura, P |
| author_facet | Priest, L Peters, JS Kukura, P |
| author_sort | Priest, L |
| collection | OXFORD |
| description | Our ability to detect, image, and quantify nanoscopic objects and molecules with visible light has undergone dramatic improvements over the past few decades. While fluorescence has historically been the go-to contrast mechanism for ultrasensitive light microscopy due to its superior background suppression and specificity, recent developments based on light scattering have reached single-molecule sensitivity. They also have the advantages of universal applicability and the ability to obtain information about the species of interest beyond its presence and location. Many of the recent advances are driven by novel approaches to illumination, detection, and background suppression, all aimed at isolating and maximizing the signal of interest. Here, we review these developments grouped according to the basic principles used, namely darkfield imaging, interferometric detection, and surface plasmon resonance microscopy. |
| first_indexed | 2024-03-07T00:28:24Z |
| format | Journal article |
| id | oxford-uuid:7eed59ab-dd80-41ef-ab3e-14f020ff6d8a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:28:24Z |
| publishDate | 2021 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:7eed59ab-dd80-41ef-ab3e-14f020ff6d8a2022-03-26T21:13:24ZScattering-based light microscopy: From metal nanoparticles to single proteinsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7eed59ab-dd80-41ef-ab3e-14f020ff6d8aEnglishSymplectic ElementsAmerican Chemical Society2021Priest, LPeters, JSKukura, POur ability to detect, image, and quantify nanoscopic objects and molecules with visible light has undergone dramatic improvements over the past few decades. While fluorescence has historically been the go-to contrast mechanism for ultrasensitive light microscopy due to its superior background suppression and specificity, recent developments based on light scattering have reached single-molecule sensitivity. They also have the advantages of universal applicability and the ability to obtain information about the species of interest beyond its presence and location. Many of the recent advances are driven by novel approaches to illumination, detection, and background suppression, all aimed at isolating and maximizing the signal of interest. Here, we review these developments grouped according to the basic principles used, namely darkfield imaging, interferometric detection, and surface plasmon resonance microscopy. |
| spellingShingle | Priest, L Peters, JS Kukura, P Scattering-based light microscopy: From metal nanoparticles to single proteins |
| title | Scattering-based light microscopy: From metal nanoparticles to single proteins |
| title_full | Scattering-based light microscopy: From metal nanoparticles to single proteins |
| title_fullStr | Scattering-based light microscopy: From metal nanoparticles to single proteins |
| title_full_unstemmed | Scattering-based light microscopy: From metal nanoparticles to single proteins |
| title_short | Scattering-based light microscopy: From metal nanoparticles to single proteins |
| title_sort | scattering based light microscopy from metal nanoparticles to single proteins |
| work_keys_str_mv | AT priestl scatteringbasedlightmicroscopyfrommetalnanoparticlestosingleproteins AT petersjs scatteringbasedlightmicroscopyfrommetalnanoparticlestosingleproteins AT kukurap scatteringbasedlightmicroscopyfrommetalnanoparticlestosingleproteins |