Antiviral responses induced by Tdap-IPV vaccination are associated with persistent humoral immunity to Bordetella pertussis
Many countries continue to experience pertussis epidemics despite widespread vaccination. Waning protection after booster vaccination has highlighted the need for a better understanding of the immunological factors that promote durable protection. Here we apply systems vaccinology to investigate ant...
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Sprache: | English |
| Veröffentlicht: |
Nature Research
2024
|
| _version_ | 1826313079511580672 |
|---|---|
| author | Gillard, J Suffiotti, M Brazda, P Venkatasubramanian, PB Versteegen, P de Jonge, MI Kelly, D Bibi, S Pinto, MV Simonetti, E Babiceanu, M Kettring, A Teodosio, C de Groot, R Berbers, G Stunnenberg, HG Schanen, B Fenwick, C Huynen, MA Diavatopoulos, DA |
| author_facet | Gillard, J Suffiotti, M Brazda, P Venkatasubramanian, PB Versteegen, P de Jonge, MI Kelly, D Bibi, S Pinto, MV Simonetti, E Babiceanu, M Kettring, A Teodosio, C de Groot, R Berbers, G Stunnenberg, HG Schanen, B Fenwick, C Huynen, MA Diavatopoulos, DA |
| author_sort | Gillard, J |
| collection | OXFORD |
| description | Many countries continue to experience pertussis epidemics despite widespread vaccination. Waning protection after booster vaccination has highlighted the need for a better understanding of the immunological factors that promote durable protection. Here we apply systems vaccinology to investigate antibody responses in adolescents in the Netherlands (N = 14; NL) and the United Kingdom (N = 12; UK) receiving a tetanus-diphtheria-acellular pertussis-inactivated poliovirus (Tdap-IPV) vaccine. We report that early antiviral and interferon gene expression signatures in blood correlate to persistence of pertussis-specific antibody responses. Single-cell analyses of the innate response identified monocytes and myeloid dendritic cells (MoDC) as principal responders that upregulate antiviral gene expression and type-I interferon cytokine production. With public data, we show that Tdap vaccination stimulates significantly lower antiviral/type-I interferon responses than Tdap-IPV, suggesting that IPV may promote antiviral gene expression. Subsequent in vitro stimulation experiments demonstrate TLR-dependent, IPV-specific activation of the pro-inflammatory p38 MAP kinase pathway in MoDCs. Together, our data provide insights into the molecular host response to pertussis booster vaccination and demonstrate that IPV enhances innate immune activity associated with persistent, pertussis-specific antibody responses. |
| first_indexed | 2024-09-25T04:07:08Z |
| format | Journal article |
| id | oxford-uuid:7ef6e508-6d20-446d-81c8-301a11356dcb |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-09-25T04:07:08Z |
| publishDate | 2024 |
| publisher | Nature Research |
| record_format | dspace |
| spelling | oxford-uuid:7ef6e508-6d20-446d-81c8-301a11356dcb2024-05-30T10:31:26ZAntiviral responses induced by Tdap-IPV vaccination are associated with persistent humoral immunity to Bordetella pertussisJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7ef6e508-6d20-446d-81c8-301a11356dcbEnglishJisc Publications RouterNature Research2024Gillard, JSuffiotti, MBrazda, PVenkatasubramanian, PBVersteegen, Pde Jonge, MIKelly, DBibi, SPinto, MVSimonetti, EBabiceanu, MKettring, ATeodosio, Cde Groot, RBerbers, GStunnenberg, HGSchanen, BFenwick, CHuynen, MADiavatopoulos, DAMany countries continue to experience pertussis epidemics despite widespread vaccination. Waning protection after booster vaccination has highlighted the need for a better understanding of the immunological factors that promote durable protection. Here we apply systems vaccinology to investigate antibody responses in adolescents in the Netherlands (N = 14; NL) and the United Kingdom (N = 12; UK) receiving a tetanus-diphtheria-acellular pertussis-inactivated poliovirus (Tdap-IPV) vaccine. We report that early antiviral and interferon gene expression signatures in blood correlate to persistence of pertussis-specific antibody responses. Single-cell analyses of the innate response identified monocytes and myeloid dendritic cells (MoDC) as principal responders that upregulate antiviral gene expression and type-I interferon cytokine production. With public data, we show that Tdap vaccination stimulates significantly lower antiviral/type-I interferon responses than Tdap-IPV, suggesting that IPV may promote antiviral gene expression. Subsequent in vitro stimulation experiments demonstrate TLR-dependent, IPV-specific activation of the pro-inflammatory p38 MAP kinase pathway in MoDCs. Together, our data provide insights into the molecular host response to pertussis booster vaccination and demonstrate that IPV enhances innate immune activity associated with persistent, pertussis-specific antibody responses. |
| spellingShingle | Gillard, J Suffiotti, M Brazda, P Venkatasubramanian, PB Versteegen, P de Jonge, MI Kelly, D Bibi, S Pinto, MV Simonetti, E Babiceanu, M Kettring, A Teodosio, C de Groot, R Berbers, G Stunnenberg, HG Schanen, B Fenwick, C Huynen, MA Diavatopoulos, DA Antiviral responses induced by Tdap-IPV vaccination are associated with persistent humoral immunity to Bordetella pertussis |
| title | Antiviral responses induced by Tdap-IPV vaccination are associated with persistent humoral immunity to Bordetella pertussis |
| title_full | Antiviral responses induced by Tdap-IPV vaccination are associated with persistent humoral immunity to Bordetella pertussis |
| title_fullStr | Antiviral responses induced by Tdap-IPV vaccination are associated with persistent humoral immunity to Bordetella pertussis |
| title_full_unstemmed | Antiviral responses induced by Tdap-IPV vaccination are associated with persistent humoral immunity to Bordetella pertussis |
| title_short | Antiviral responses induced by Tdap-IPV vaccination are associated with persistent humoral immunity to Bordetella pertussis |
| title_sort | antiviral responses induced by tdap ipv vaccination are associated with persistent humoral immunity to bordetella pertussis |
| work_keys_str_mv | AT gillardj antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT suffiottim antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT brazdap antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT venkatasubramanianpb antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT versteegenp antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT dejongemi antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT kellyd antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT bibis antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT pintomv antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT simonettie antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT babiceanum antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT kettringa antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT teodosioc antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT degrootr antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT berbersg antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT stunnenberghg antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT schanenb antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT fenwickc antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT huynenma antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis AT diavatopoulosda antiviralresponsesinducedbytdapipvvaccinationareassociatedwithpersistenthumoralimmunitytobordetellapertussis |