Global phylogenetic analysis of Escherichia coli and plasmids carrying the mcr-1 gene indicates bacterial diversity but plasmid restriction
To understand the dynamics behind the worldwide spread of the mcr-1 gene, we determined the population structure of Escherichia coli and of mobile genetic elements (MGEs) carrying the mcr-1 gene. After a systematic review of the literature we included 65 E. coli whole genome sequences (WGS), adding...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
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Nature Publishing Group
2017
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| _version_ | 1797078361135120384 |
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| author | Matamoros, S van Hattem, JM Arcilla, MS Willemse, N Melles, DC Penders, J Vinh, TN Ngo, HT de Jong, MD Schultsz, C |
| author_facet | Matamoros, S van Hattem, JM Arcilla, MS Willemse, N Melles, DC Penders, J Vinh, TN Ngo, HT de Jong, MD Schultsz, C |
| author_sort | Matamoros, S |
| collection | OXFORD |
| description | To understand the dynamics behind the worldwide spread of the mcr-1 gene, we determined the population structure of Escherichia coli and of mobile genetic elements (MGEs) carrying the mcr-1 gene. After a systematic review of the literature we included 65 E. coli whole genome sequences (WGS), adding 6 recently sequenced travel related isolates, and 312 MLST profiles. We included 219 MGEs described in 7 Enterobacteriaceae species isolated from human, animal and environmental samples. Despite a high overall diversity, 2 lineages were observed in the E. coli population that may function as reservoirs of the mcr-1 gene, the largest of which was linked to ST10, a sequence type known for its ubiquity in human faecal samples and in food samples. No genotypic clustering by geographical origin or isolation source was observed. Amongst a total of 13 plasmid incompatibility types, the IncI2, IncX4 and IncHI2 plasmids accounted for more than 90% of MGEs carrying the mcr-1 gene. We observed significant geographical clustering with regional spread of IncHI2 plasmids in Europe and IncI2 in Asia. These findings point towards promiscuous spread of the mcr-1 gene by efficient horizontal gene transfer dominated by a limited number of plasmid incompatibility types. |
| first_indexed | 2024-03-07T00:30:50Z |
| format | Journal article |
| id | oxford-uuid:7fbe5aee-cf6b-4b6f-afde-80b65abb8751 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T00:30:50Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | oxford-uuid:7fbe5aee-cf6b-4b6f-afde-80b65abb87512022-03-26T21:18:51ZGlobal phylogenetic analysis of Escherichia coli and plasmids carrying the mcr-1 gene indicates bacterial diversity but plasmid restrictionJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7fbe5aee-cf6b-4b6f-afde-80b65abb8751Symplectic Elements at OxfordNature Publishing Group2017Matamoros, Svan Hattem, JMArcilla, MSWillemse, NMelles, DCPenders, JVinh, TNNgo, HTde Jong, MDSchultsz, CTo understand the dynamics behind the worldwide spread of the mcr-1 gene, we determined the population structure of Escherichia coli and of mobile genetic elements (MGEs) carrying the mcr-1 gene. After a systematic review of the literature we included 65 E. coli whole genome sequences (WGS), adding 6 recently sequenced travel related isolates, and 312 MLST profiles. We included 219 MGEs described in 7 Enterobacteriaceae species isolated from human, animal and environmental samples. Despite a high overall diversity, 2 lineages were observed in the E. coli population that may function as reservoirs of the mcr-1 gene, the largest of which was linked to ST10, a sequence type known for its ubiquity in human faecal samples and in food samples. No genotypic clustering by geographical origin or isolation source was observed. Amongst a total of 13 plasmid incompatibility types, the IncI2, IncX4 and IncHI2 plasmids accounted for more than 90% of MGEs carrying the mcr-1 gene. We observed significant geographical clustering with regional spread of IncHI2 plasmids in Europe and IncI2 in Asia. These findings point towards promiscuous spread of the mcr-1 gene by efficient horizontal gene transfer dominated by a limited number of plasmid incompatibility types. |
| spellingShingle | Matamoros, S van Hattem, JM Arcilla, MS Willemse, N Melles, DC Penders, J Vinh, TN Ngo, HT de Jong, MD Schultsz, C Global phylogenetic analysis of Escherichia coli and plasmids carrying the mcr-1 gene indicates bacterial diversity but plasmid restriction |
| title | Global phylogenetic analysis of Escherichia coli and plasmids carrying the mcr-1 gene indicates bacterial diversity but plasmid restriction |
| title_full | Global phylogenetic analysis of Escherichia coli and plasmids carrying the mcr-1 gene indicates bacterial diversity but plasmid restriction |
| title_fullStr | Global phylogenetic analysis of Escherichia coli and plasmids carrying the mcr-1 gene indicates bacterial diversity but plasmid restriction |
| title_full_unstemmed | Global phylogenetic analysis of Escherichia coli and plasmids carrying the mcr-1 gene indicates bacterial diversity but plasmid restriction |
| title_short | Global phylogenetic analysis of Escherichia coli and plasmids carrying the mcr-1 gene indicates bacterial diversity but plasmid restriction |
| title_sort | global phylogenetic analysis of escherichia coli and plasmids carrying the mcr 1 gene indicates bacterial diversity but plasmid restriction |
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