The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant
A key step in meiotic recombination involves the nucleolytic resolution of Holliday junctions to generate crossovers. Although the enzyme that performs this function in human cells is presently unknown, recent studies led to the identification of the XPG-family endonuclease GEN1 that promotes Hollid...
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| Format: | Journal article |
| Language: | English |
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Oxford University Press
2010
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| author | Lorenz, A West, S Whitby, M |
| author_facet | Lorenz, A West, S Whitby, M |
| author_sort | Lorenz, A |
| collection | OXFORD |
| description | A key step in meiotic recombination involves the nucleolytic resolution of Holliday junctions to generate crossovers. Although the enzyme that performs this function in human cells is presently unknown, recent studies led to the identification of the XPG-family endonuclease GEN1 that promotes Holliday junction resolution <em>in vitro</em>, suggesting that it may perform a related function <em>in vivo</em>. Here, we show that ectopic expression of GEN1 in fission yeast mus81Δ strains result in Holliday junction resolution and crossover formation during meiosis. |
| first_indexed | 2024-03-07T00:31:32Z |
| format | Journal article |
| id | oxford-uuid:7ff5d597-4394-4c83-85d6-f98761b93b6e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:31:32Z |
| publishDate | 2010 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:7ff5d597-4394-4c83-85d6-f98761b93b6e2022-03-26T21:20:16ZThe human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutantJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7ff5d597-4394-4c83-85d6-f98761b93b6eBiochemistryEnglishOxford University Research Archive - ValetOxford University Press2010Lorenz, AWest, SWhitby, MA key step in meiotic recombination involves the nucleolytic resolution of Holliday junctions to generate crossovers. Although the enzyme that performs this function in human cells is presently unknown, recent studies led to the identification of the XPG-family endonuclease GEN1 that promotes Holliday junction resolution <em>in vitro</em>, suggesting that it may perform a related function <em>in vivo</em>. Here, we show that ectopic expression of GEN1 in fission yeast mus81Δ strains result in Holliday junction resolution and crossover formation during meiosis. |
| spellingShingle | Biochemistry Lorenz, A West, S Whitby, M The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant |
| title | The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant |
| title_full | The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant |
| title_fullStr | The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant |
| title_full_unstemmed | The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant |
| title_short | The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant |
| title_sort | human holliday junction resolvase gen1 rescues the meiotic phenotype of a schizosaccharomyces pombe mus81 mutant |
| topic | Biochemistry |
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