Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells.
BACKGROUND: Although substantial evidence suggests that T cells are important in the pathogenesis of atopic dermatitis (AD), little is known of the differentiation status of CD4+ T cells specific for common environmental allergens. OBJECTIVE: To determine the frequency, differentiation phenotype, an...
| Päätekijät: | , , |
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| Aineistotyyppi: | Journal article |
| Kieli: | English |
| Julkaistu: |
2006
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| _version_ | 1826281673684156416 |
|---|---|
| author | Bateman, E Ardern-Jones, MR Ogg, G |
| author_facet | Bateman, E Ardern-Jones, MR Ogg, G |
| author_sort | Bateman, E |
| collection | OXFORD |
| description | BACKGROUND: Although substantial evidence suggests that T cells are important in the pathogenesis of atopic dermatitis (AD), little is known of the differentiation status of CD4+ T cells specific for common environmental allergens. OBJECTIVE: To determine the frequency, differentiation phenotype, and function of circulating allergen-specific CD4+ T cells in adult individuals with severe persistent AD and controls. METHODS: Using tetrameric complexes of an HLA DRB1*0101 restricted epitope from Fel d 1, the major IgE-reactive component of cat dander, we studied ex vivo and cultured T-cell frequency and phenotype in individuals with AD and healthy controls. Cytokine secretion was measured by ex vivo and cultured IFN-gamma, IL-4, and IL-10 enzyme linked immuno-spot analysis. RESULTS: Ex vivo Fel d 1-specific DRB1*0101-restricted CD4+ T cells express high levels of CCR7, CD62L, CD27, and CD28 and proportionately low levels of tissue-specific homing receptors and TH1 and TH2 cytokine production, placing the cells largely within the central memory subgroup. CONCLUSION: Circulating Fel d 1-specific DRB1*0101-restricted CD4+ T cells maintain central memory capacity, consistent with a potential to contribute to persisting clinical atopic disease. CLINICAL IMPLICATIONS: Persisting central memory characteristics of allergen-specific CD4+ T cells in individuals with AD may contribute to chronic disease. |
| first_indexed | 2024-03-07T00:32:20Z |
| format | Journal article |
| id | oxford-uuid:80404e88-b5ec-4551-942b-1d99f7e5a5a7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:32:20Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:80404e88-b5ec-4551-942b-1d99f7e5a5a72022-03-26T21:21:59ZPersistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:80404e88-b5ec-4551-942b-1d99f7e5a5a7EnglishSymplectic Elements at Oxford2006Bateman, EArdern-Jones, MROgg, GBACKGROUND: Although substantial evidence suggests that T cells are important in the pathogenesis of atopic dermatitis (AD), little is known of the differentiation status of CD4+ T cells specific for common environmental allergens. OBJECTIVE: To determine the frequency, differentiation phenotype, and function of circulating allergen-specific CD4+ T cells in adult individuals with severe persistent AD and controls. METHODS: Using tetrameric complexes of an HLA DRB1*0101 restricted epitope from Fel d 1, the major IgE-reactive component of cat dander, we studied ex vivo and cultured T-cell frequency and phenotype in individuals with AD and healthy controls. Cytokine secretion was measured by ex vivo and cultured IFN-gamma, IL-4, and IL-10 enzyme linked immuno-spot analysis. RESULTS: Ex vivo Fel d 1-specific DRB1*0101-restricted CD4+ T cells express high levels of CCR7, CD62L, CD27, and CD28 and proportionately low levels of tissue-specific homing receptors and TH1 and TH2 cytokine production, placing the cells largely within the central memory subgroup. CONCLUSION: Circulating Fel d 1-specific DRB1*0101-restricted CD4+ T cells maintain central memory capacity, consistent with a potential to contribute to persisting clinical atopic disease. CLINICAL IMPLICATIONS: Persisting central memory characteristics of allergen-specific CD4+ T cells in individuals with AD may contribute to chronic disease. |
| spellingShingle | Bateman, E Ardern-Jones, MR Ogg, G Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells. |
| title | Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells. |
| title_full | Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells. |
| title_fullStr | Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells. |
| title_full_unstemmed | Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells. |
| title_short | Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells. |
| title_sort | persistent central memory phenotype of circulating fel d 1 peptide drb1 0101 tetramer binding cd4 t cells |
| work_keys_str_mv | AT batemane persistentcentralmemoryphenotypeofcirculatingfeld1peptidedrb10101tetramerbindingcd4tcells AT ardernjonesmr persistentcentralmemoryphenotypeofcirculatingfeld1peptidedrb10101tetramerbindingcd4tcells AT oggg persistentcentralmemoryphenotypeofcirculatingfeld1peptidedrb10101tetramerbindingcd4tcells |