Adaptive optics enables 3D STED microscopy in aberrating specimens.
Stimulated emission depletion (STED) microscopy allows fluorescence far-field imaging with diffraction-unlimited resolution. Unfortunately, extending this technique to three-dimensional (3D) imaging of thick specimens has been inhibited by sample-induced aberrations. Here we present the first implem...
| Main Authors: | , , , |
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| פורמט: | Journal article |
| שפה: | English |
| יצא לאור: |
2012
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| _version_ | 1826281686957031424 |
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| author | Gould, T Burke, D Bewersdorf, J Booth, M |
| author_facet | Gould, T Burke, D Bewersdorf, J Booth, M |
| author_sort | Gould, T |
| collection | OXFORD |
| description | Stimulated emission depletion (STED) microscopy allows fluorescence far-field imaging with diffraction-unlimited resolution. Unfortunately, extending this technique to three-dimensional (3D) imaging of thick specimens has been inhibited by sample-induced aberrations. Here we present the first implementation of adaptive optics in STED microscopy to allow 3D super-resolution imaging in strongly aberrated imaging conditions, such as those introduced by thick biological tissue. |
| first_indexed | 2024-03-07T00:32:33Z |
| format | Journal article |
| id | oxford-uuid:8052ce4a-9a16-410a-8ea6-de64e699b7d9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:32:33Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:8052ce4a-9a16-410a-8ea6-de64e699b7d92022-03-26T21:22:28ZAdaptive optics enables 3D STED microscopy in aberrating specimens.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8052ce4a-9a16-410a-8ea6-de64e699b7d9EnglishSymplectic Elements at Oxford2012Gould, TBurke, DBewersdorf, JBooth, MStimulated emission depletion (STED) microscopy allows fluorescence far-field imaging with diffraction-unlimited resolution. Unfortunately, extending this technique to three-dimensional (3D) imaging of thick specimens has been inhibited by sample-induced aberrations. Here we present the first implementation of adaptive optics in STED microscopy to allow 3D super-resolution imaging in strongly aberrated imaging conditions, such as those introduced by thick biological tissue. |
| spellingShingle | Gould, T Burke, D Bewersdorf, J Booth, M Adaptive optics enables 3D STED microscopy in aberrating specimens. |
| title | Adaptive optics enables 3D STED microscopy in aberrating specimens. |
| title_full | Adaptive optics enables 3D STED microscopy in aberrating specimens. |
| title_fullStr | Adaptive optics enables 3D STED microscopy in aberrating specimens. |
| title_full_unstemmed | Adaptive optics enables 3D STED microscopy in aberrating specimens. |
| title_short | Adaptive optics enables 3D STED microscopy in aberrating specimens. |
| title_sort | adaptive optics enables 3d sted microscopy in aberrating specimens |
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