Cone photoreceptor neuroprotection conferred by CNTF in a novel in vivo model of battlefield retinal laser injury.
PURPOSE: To develop a reproducible laboratory model to simulate a battlefield foveal laser injury and to test potential neuroprotective effects of a single injection treatment that might be administered in a military setting. METHODS: Frequency-doubled 532-nm Nd:YAG laser was used to induce a thres...
| المؤلفون الرئيسيون: | , , , , , , |
|---|---|
| التنسيق: | Journal article |
| اللغة: | English |
| منشور في: |
2013
|
| _version_ | 1826281700875829248 |
|---|---|
| author | Aslam, SA Davies, W Singh, MS Charbel Issa, P Barnard, A Scott, R Maclaren, R |
| author_facet | Aslam, SA Davies, W Singh, MS Charbel Issa, P Barnard, A Scott, R Maclaren, R |
| author_sort | Aslam, SA |
| collection | OXFORD |
| description | PURPOSE: To develop a reproducible laboratory model to simulate a battlefield foveal laser injury and to test potential neuroprotective effects of a single injection treatment that might be administered in a military setting. METHODS: Frequency-doubled 532-nm Nd:YAG laser was used to induce a threshold retinal injury bilaterally in transgenic reporter mice that have fluorescent cones. Intravitreal injection of ciliary neurotrophic factor (CNTF) was then administered to the lasered eye and compared with a contralateral sham injection of saline. The effect on fluorescent cone cell survival was quantified using a confocal scanning laser ophthalmoscope (cSLO), TUNEL assays, and quantitative real-time PCR (qPCR). RESULTS: At 3 weeks post-laser, cSLO imaging showed that the proportion of surviving cones expressing green fluorescent protein (GFP) was greater in CNTF-treated (54.1 ± 5.15% of baseline count) than in sham-injected eyes (28.7 ± 4.4%), which was accompanied by a reduction in TUNEL-positive cells. This difference in cone survival persisted at the 6-week point (treated, 39.6 ± 3.2% versus sham, 18.0 ± 3.8%). These changes were accompanied by a reduction in TUNEL-positive cells. The Bcl-2/Bax ratio was increased in CNTF-treated eyes at 1 week postlaser exposure relative to controls. CONCLUSIONS: A single intravitreal injection of CNTF protein was shown to improve cone survival when administered immediately after laser exposure. Similar treatments with CNTF might also have a role in attenuating retinal laser damage sustained by combat personnel in the military setting. |
| first_indexed | 2024-03-07T00:32:45Z |
| format | Journal article |
| id | oxford-uuid:8061c44d-c647-4b9c-bb43-796fe64bb62d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:32:45Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:8061c44d-c647-4b9c-bb43-796fe64bb62d2022-03-26T21:22:58ZCone photoreceptor neuroprotection conferred by CNTF in a novel in vivo model of battlefield retinal laser injury.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8061c44d-c647-4b9c-bb43-796fe64bb62dEnglishSymplectic Elements at Oxford2013Aslam, SADavies, WSingh, MSCharbel Issa, PBarnard, AScott, RMaclaren, R PURPOSE: To develop a reproducible laboratory model to simulate a battlefield foveal laser injury and to test potential neuroprotective effects of a single injection treatment that might be administered in a military setting. METHODS: Frequency-doubled 532-nm Nd:YAG laser was used to induce a threshold retinal injury bilaterally in transgenic reporter mice that have fluorescent cones. Intravitreal injection of ciliary neurotrophic factor (CNTF) was then administered to the lasered eye and compared with a contralateral sham injection of saline. The effect on fluorescent cone cell survival was quantified using a confocal scanning laser ophthalmoscope (cSLO), TUNEL assays, and quantitative real-time PCR (qPCR). RESULTS: At 3 weeks post-laser, cSLO imaging showed that the proportion of surviving cones expressing green fluorescent protein (GFP) was greater in CNTF-treated (54.1 ± 5.15% of baseline count) than in sham-injected eyes (28.7 ± 4.4%), which was accompanied by a reduction in TUNEL-positive cells. This difference in cone survival persisted at the 6-week point (treated, 39.6 ± 3.2% versus sham, 18.0 ± 3.8%). These changes were accompanied by a reduction in TUNEL-positive cells. The Bcl-2/Bax ratio was increased in CNTF-treated eyes at 1 week postlaser exposure relative to controls. CONCLUSIONS: A single intravitreal injection of CNTF protein was shown to improve cone survival when administered immediately after laser exposure. Similar treatments with CNTF might also have a role in attenuating retinal laser damage sustained by combat personnel in the military setting. |
| spellingShingle | Aslam, SA Davies, W Singh, MS Charbel Issa, P Barnard, A Scott, R Maclaren, R Cone photoreceptor neuroprotection conferred by CNTF in a novel in vivo model of battlefield retinal laser injury. |
| title | Cone photoreceptor neuroprotection conferred by CNTF in a novel in vivo model of battlefield retinal laser injury. |
| title_full | Cone photoreceptor neuroprotection conferred by CNTF in a novel in vivo model of battlefield retinal laser injury. |
| title_fullStr | Cone photoreceptor neuroprotection conferred by CNTF in a novel in vivo model of battlefield retinal laser injury. |
| title_full_unstemmed | Cone photoreceptor neuroprotection conferred by CNTF in a novel in vivo model of battlefield retinal laser injury. |
| title_short | Cone photoreceptor neuroprotection conferred by CNTF in a novel in vivo model of battlefield retinal laser injury. |
| title_sort | cone photoreceptor neuroprotection conferred by cntf in a novel in vivo model of battlefield retinal laser injury |
| work_keys_str_mv | AT aslamsa conephotoreceptorneuroprotectionconferredbycntfinanovelinvivomodelofbattlefieldretinallaserinjury AT daviesw conephotoreceptorneuroprotectionconferredbycntfinanovelinvivomodelofbattlefieldretinallaserinjury AT singhms conephotoreceptorneuroprotectionconferredbycntfinanovelinvivomodelofbattlefieldretinallaserinjury AT charbelissap conephotoreceptorneuroprotectionconferredbycntfinanovelinvivomodelofbattlefieldretinallaserinjury AT barnarda conephotoreceptorneuroprotectionconferredbycntfinanovelinvivomodelofbattlefieldretinallaserinjury AT scottr conephotoreceptorneuroprotectionconferredbycntfinanovelinvivomodelofbattlefieldretinallaserinjury AT maclarenr conephotoreceptorneuroprotectionconferredbycntfinanovelinvivomodelofbattlefieldretinallaserinjury |