Two-dimensional crystallization of a histidine-tagged protein on monolayers of fluidity-enhanced Ni2+-chelating lipids
Protein two-dimensional (2D) crystallization on lipid monolayers is a powerful method for structure determination. This method has been extended using the specific and strong interaction between histidine residues (of an overexpressed protein) and Ni2+ ions tethered at the headgroup of synthetic lip...
Main Authors: | Courty, S, Lebeau, L, Martel, L, Lenne, P, Balavoine, F, Dischert, W, Konovalov, O, Mioskowski, C, Legrand, J, Venien-Bryan, C |
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格式: | Journal article |
语言: | English |
出版: |
2002
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