Interleukin-6, a growth promoting cytokine, is present in human pituitary adenomas: an immunocytochemical study.
OBJECTIVE: The aim of this study was to investigate the presence of interleukin-6 (IL-6) in human pituitary adenomas. DESIGN: Immunocytochemistry was performed in 15 cases of pituitary adenomas obtained at transsphenoidal surgery. PATIENTS: Fifteen cases of pituitary adenomas were examined; these in...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1992
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| _version_ | 1797078666700652544 |
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| author | Tsagarakis, S Kontogeorgos, G Giannou, P Thalassinos, N Woolley, J Besser, G Grossman, A |
| author_facet | Tsagarakis, S Kontogeorgos, G Giannou, P Thalassinos, N Woolley, J Besser, G Grossman, A |
| author_sort | Tsagarakis, S |
| collection | OXFORD |
| description | OBJECTIVE: The aim of this study was to investigate the presence of interleukin-6 (IL-6) in human pituitary adenomas. DESIGN: Immunocytochemistry was performed in 15 cases of pituitary adenomas obtained at transsphenoidal surgery. PATIENTS: Fifteen cases of pituitary adenomas were examined; these included five GH-secreting adenomas, four prolactinomas, four ACTH-secreting adenomas and two null-cell ('functionless') adenomas. MEASUREMENTS: Each tumour was stained for the presence of IL-6 by means of the avidin-biotin-peroxidase complex (ABC) technique, and was also stained for GH, prolactin, ACTH and alpha-subunit by conventional immunocytochemistry. RESULTS: Fourteen of the 15 tumours examined, excepting one GH-secreting tumour, demonstrated clusters of IL-6 positively stained cells; the specificity of the staining was established by a significant decrease in staining in parallel sections of all tumours studied when the anti IL-6 antibody was preabsorbed with recombinant human IL-6. CONCLUSIONS: It is concluded that IL-6 immunoreactivity is present in human pituitary tumours, and is independent of the specific secretory cell product. The role of this cytokine in the pathogenesis of such tumours requires further investigation. |
| first_indexed | 2024-03-07T00:35:01Z |
| format | Journal article |
| id | oxford-uuid:811c40eb-128b-47af-97a2-96af6980e3e0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:35:01Z |
| publishDate | 1992 |
| record_format | dspace |
| spelling | oxford-uuid:811c40eb-128b-47af-97a2-96af6980e3e02022-03-26T21:28:04ZInterleukin-6, a growth promoting cytokine, is present in human pituitary adenomas: an immunocytochemical study.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:811c40eb-128b-47af-97a2-96af6980e3e0EnglishSymplectic Elements at Oxford1992Tsagarakis, SKontogeorgos, GGiannou, PThalassinos, NWoolley, JBesser, GGrossman, AOBJECTIVE: The aim of this study was to investigate the presence of interleukin-6 (IL-6) in human pituitary adenomas. DESIGN: Immunocytochemistry was performed in 15 cases of pituitary adenomas obtained at transsphenoidal surgery. PATIENTS: Fifteen cases of pituitary adenomas were examined; these included five GH-secreting adenomas, four prolactinomas, four ACTH-secreting adenomas and two null-cell ('functionless') adenomas. MEASUREMENTS: Each tumour was stained for the presence of IL-6 by means of the avidin-biotin-peroxidase complex (ABC) technique, and was also stained for GH, prolactin, ACTH and alpha-subunit by conventional immunocytochemistry. RESULTS: Fourteen of the 15 tumours examined, excepting one GH-secreting tumour, demonstrated clusters of IL-6 positively stained cells; the specificity of the staining was established by a significant decrease in staining in parallel sections of all tumours studied when the anti IL-6 antibody was preabsorbed with recombinant human IL-6. CONCLUSIONS: It is concluded that IL-6 immunoreactivity is present in human pituitary tumours, and is independent of the specific secretory cell product. The role of this cytokine in the pathogenesis of such tumours requires further investigation. |
| spellingShingle | Tsagarakis, S Kontogeorgos, G Giannou, P Thalassinos, N Woolley, J Besser, G Grossman, A Interleukin-6, a growth promoting cytokine, is present in human pituitary adenomas: an immunocytochemical study. |
| title | Interleukin-6, a growth promoting cytokine, is present in human pituitary adenomas: an immunocytochemical study. |
| title_full | Interleukin-6, a growth promoting cytokine, is present in human pituitary adenomas: an immunocytochemical study. |
| title_fullStr | Interleukin-6, a growth promoting cytokine, is present in human pituitary adenomas: an immunocytochemical study. |
| title_full_unstemmed | Interleukin-6, a growth promoting cytokine, is present in human pituitary adenomas: an immunocytochemical study. |
| title_short | Interleukin-6, a growth promoting cytokine, is present in human pituitary adenomas: an immunocytochemical study. |
| title_sort | interleukin 6 a growth promoting cytokine is present in human pituitary adenomas an immunocytochemical study |
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