Control of antigen presentation with a photoreleasable agonist peptide.
The immunological synapse is a specialized intercellular junction between a T cell and a target cell that orchestrates the engagement of receptors and ligands in space and time as a means of regulating function. Here we introduce a reagent for controlling the spatial and temporal presentation of nat...
| Hauptverfasser: | , , , |
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| Format: | Journal article |
| Sprache: | English |
| Veröffentlicht: |
2006
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| _version_ | 1826281865303031808 |
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| author | DeMond, A Starr, T Dustin, M Groves, J |
| author_facet | DeMond, A Starr, T Dustin, M Groves, J |
| author_sort | DeMond, A |
| collection | OXFORD |
| description | The immunological synapse is a specialized intercellular junction between a T cell and a target cell that orchestrates the engagement of receptors and ligands in space and time as a means of regulating function. Here we introduce a reagent for controlling the spatial and temporal presentation of natural antigen to T cells. Moth cytochrome c (88-103) peptide (MCC), an agonist to the murine T cell receptor AND when presented in the context of H2 IEk major histocompatibility complex (IEk), was synthesized with the side-chain amine of Lys99 conjugated to a photosensitive protecting group, 6-nitroveratryloxycarbonyl (NVOC). Cells plated on supported bilayers displaying mobile intercellular adhesion molecule-1 (ICAM-1) and NVOC-MCC loaded IEk did not form immunological synapses and exhibited low intracellular calcium levels, similar to cells presented with self-peptide. Irradiation with UV light was sufficient to restore agonist activity in situ. |
| first_indexed | 2024-03-07T00:35:14Z |
| format | Journal article |
| id | oxford-uuid:812cb840-fa4b-4907-9f11-4c6105114fc8 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:35:14Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:812cb840-fa4b-4907-9f11-4c6105114fc82022-03-26T21:28:32ZControl of antigen presentation with a photoreleasable agonist peptide.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:812cb840-fa4b-4907-9f11-4c6105114fc8EnglishSymplectic Elements at Oxford2006DeMond, AStarr, TDustin, MGroves, JThe immunological synapse is a specialized intercellular junction between a T cell and a target cell that orchestrates the engagement of receptors and ligands in space and time as a means of regulating function. Here we introduce a reagent for controlling the spatial and temporal presentation of natural antigen to T cells. Moth cytochrome c (88-103) peptide (MCC), an agonist to the murine T cell receptor AND when presented in the context of H2 IEk major histocompatibility complex (IEk), was synthesized with the side-chain amine of Lys99 conjugated to a photosensitive protecting group, 6-nitroveratryloxycarbonyl (NVOC). Cells plated on supported bilayers displaying mobile intercellular adhesion molecule-1 (ICAM-1) and NVOC-MCC loaded IEk did not form immunological synapses and exhibited low intracellular calcium levels, similar to cells presented with self-peptide. Irradiation with UV light was sufficient to restore agonist activity in situ. |
| spellingShingle | DeMond, A Starr, T Dustin, M Groves, J Control of antigen presentation with a photoreleasable agonist peptide. |
| title | Control of antigen presentation with a photoreleasable agonist peptide. |
| title_full | Control of antigen presentation with a photoreleasable agonist peptide. |
| title_fullStr | Control of antigen presentation with a photoreleasable agonist peptide. |
| title_full_unstemmed | Control of antigen presentation with a photoreleasable agonist peptide. |
| title_short | Control of antigen presentation with a photoreleasable agonist peptide. |
| title_sort | control of antigen presentation with a photoreleasable agonist peptide |
| work_keys_str_mv | AT demonda controlofantigenpresentationwithaphotoreleasableagonistpeptide AT starrt controlofantigenpresentationwithaphotoreleasableagonistpeptide AT dustinm controlofantigenpresentationwithaphotoreleasableagonistpeptide AT grovesj controlofantigenpresentationwithaphotoreleasableagonistpeptide |