RAISED LEVELS OF TUMOR-NECROSIS-FACTOR-ALPHA (TNF-ALPHA) IN CARDIAC TRANSPLANT PATIENTS

Enzyme immunoassay is used to probe serum and plasma levels of tumor-necrosis factor-alpha (TNFα) in human heart transplant patients during the first few months post-transplantation. Detectability of TNFα was increased by heat-pretreatment of serum (56°C for 30 minutes), in order to inactivate inhib...

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Main Authors: Sabokbar, A, Horton, J, Bowler, K, Manning, R, Meager, A, Dark, J
Format: Journal article
Published: 1993
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author Sabokbar, A
Horton, J
Bowler, K
Manning, R
Meager, A
Dark, J
author_facet Sabokbar, A
Horton, J
Bowler, K
Manning, R
Meager, A
Dark, J
author_sort Sabokbar, A
collection OXFORD
description Enzyme immunoassay is used to probe serum and plasma levels of tumor-necrosis factor-alpha (TNFα) in human heart transplant patients during the first few months post-transplantation. Detectability of TNFα was increased by heat-pretreatment of serum (56°C for 30 minutes), in order to inactivate inhibitors of the immunoassay present in the sera. In EDTA-treated plasma samples such treatment was not necessary, thus suggesting the possible involvement of complement in such inhibition. Compared with samples from control (healthy) volunteers, transplant patients frequently displayed elevated levels of TNFα, especially in the first few weeks post-transplantation. However, increased cytokine levels did not correlate with histologically (biopsy)-determined rejection episodes. We were unable to detect interleukin-6 (IL-6) in plasma samples of heart transplant patients.
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spelling oxford-uuid:8132fd60-1ed2-4990-837e-3a09898e4eff2022-03-26T21:28:48ZRAISED LEVELS OF TUMOR-NECROSIS-FACTOR-ALPHA (TNF-ALPHA) IN CARDIAC TRANSPLANT PATIENTSJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8132fd60-1ed2-4990-837e-3a09898e4effSymplectic Elements at Oxford1993Sabokbar, AHorton, JBowler, KManning, RMeager, ADark, JEnzyme immunoassay is used to probe serum and plasma levels of tumor-necrosis factor-alpha (TNFα) in human heart transplant patients during the first few months post-transplantation. Detectability of TNFα was increased by heat-pretreatment of serum (56°C for 30 minutes), in order to inactivate inhibitors of the immunoassay present in the sera. In EDTA-treated plasma samples such treatment was not necessary, thus suggesting the possible involvement of complement in such inhibition. Compared with samples from control (healthy) volunteers, transplant patients frequently displayed elevated levels of TNFα, especially in the first few weeks post-transplantation. However, increased cytokine levels did not correlate with histologically (biopsy)-determined rejection episodes. We were unable to detect interleukin-6 (IL-6) in plasma samples of heart transplant patients.
spellingShingle Sabokbar, A
Horton, J
Bowler, K
Manning, R
Meager, A
Dark, J
RAISED LEVELS OF TUMOR-NECROSIS-FACTOR-ALPHA (TNF-ALPHA) IN CARDIAC TRANSPLANT PATIENTS
title RAISED LEVELS OF TUMOR-NECROSIS-FACTOR-ALPHA (TNF-ALPHA) IN CARDIAC TRANSPLANT PATIENTS
title_full RAISED LEVELS OF TUMOR-NECROSIS-FACTOR-ALPHA (TNF-ALPHA) IN CARDIAC TRANSPLANT PATIENTS
title_fullStr RAISED LEVELS OF TUMOR-NECROSIS-FACTOR-ALPHA (TNF-ALPHA) IN CARDIAC TRANSPLANT PATIENTS
title_full_unstemmed RAISED LEVELS OF TUMOR-NECROSIS-FACTOR-ALPHA (TNF-ALPHA) IN CARDIAC TRANSPLANT PATIENTS
title_short RAISED LEVELS OF TUMOR-NECROSIS-FACTOR-ALPHA (TNF-ALPHA) IN CARDIAC TRANSPLANT PATIENTS
title_sort raised levels of tumor necrosis factor alpha tnf alpha in cardiac transplant patients
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AT hortonj raisedlevelsoftumornecrosisfactoralphatnfalphaincardiactransplantpatients
AT bowlerk raisedlevelsoftumornecrosisfactoralphatnfalphaincardiactransplantpatients
AT manningr raisedlevelsoftumornecrosisfactoralphatnfalphaincardiactransplantpatients
AT meagera raisedlevelsoftumornecrosisfactoralphatnfalphaincardiactransplantpatients
AT darkj raisedlevelsoftumornecrosisfactoralphatnfalphaincardiactransplantpatients