Identification and characterization of a novel siglec, siglec-7, expressed by human natural killer cells and monocytes.
We describe the characterization of sialic acid-binding Ig-like lectin-7 (siglec-7), a novel member of the siglec subgroup of the immunoglobulin superfamily. A full-length cDNA encoding siglec-7 was isolated from a human primary dendritic cell cDNA library. Siglec-7 is predicted to contain three ext...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1999
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| _version_ | 1797078762181885952 |
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| author | Nicoll, G Ni, J Liu, D Klenerman, P Munday, J Dubock, S Mattei, MG Crocker, P |
| author_facet | Nicoll, G Ni, J Liu, D Klenerman, P Munday, J Dubock, S Mattei, MG Crocker, P |
| author_sort | Nicoll, G |
| collection | OXFORD |
| description | We describe the characterization of sialic acid-binding Ig-like lectin-7 (siglec-7), a novel member of the siglec subgroup of the immunoglobulin superfamily. A full-length cDNA encoding siglec-7 was isolated from a human primary dendritic cell cDNA library. Siglec-7 is predicted to contain three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs. Overall, siglec-7 exhibited a high degree of sequence similarity to genes encoding CD33 (siglec-3), siglec-5, OBBP1/siglec-6, and OBBP-like protein and mapped to the same region on chromosome 19q13.3. When siglec-7 was expressed on COS or Chinese hamster ovary cells, it was able to mediate high levels of sialic acid-dependent binding to human erythrocytes and soluble sialoglycoconjugates, suggesting that it may be involved in cell-cell interactions. Among human peripheral blood leukocytes, siglec-7 was found to be present at low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8(+) T cells. Immunoprecipitation experiments indicated that siglec-7 is expressed as a monomer of approximately 65 kDa. |
| first_indexed | 2024-03-07T00:36:21Z |
| format | Journal article |
| id | oxford-uuid:818b9d93-b610-409d-8b6b-041da58a9f54 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:36:21Z |
| publishDate | 1999 |
| record_format | dspace |
| spelling | oxford-uuid:818b9d93-b610-409d-8b6b-041da58a9f542022-03-26T21:31:01ZIdentification and characterization of a novel siglec, siglec-7, expressed by human natural killer cells and monocytes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:818b9d93-b610-409d-8b6b-041da58a9f54EnglishSymplectic Elements at Oxford1999Nicoll, GNi, JLiu, DKlenerman, PMunday, JDubock, SMattei, MGCrocker, PWe describe the characterization of sialic acid-binding Ig-like lectin-7 (siglec-7), a novel member of the siglec subgroup of the immunoglobulin superfamily. A full-length cDNA encoding siglec-7 was isolated from a human primary dendritic cell cDNA library. Siglec-7 is predicted to contain three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs. Overall, siglec-7 exhibited a high degree of sequence similarity to genes encoding CD33 (siglec-3), siglec-5, OBBP1/siglec-6, and OBBP-like protein and mapped to the same region on chromosome 19q13.3. When siglec-7 was expressed on COS or Chinese hamster ovary cells, it was able to mediate high levels of sialic acid-dependent binding to human erythrocytes and soluble sialoglycoconjugates, suggesting that it may be involved in cell-cell interactions. Among human peripheral blood leukocytes, siglec-7 was found to be present at low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8(+) T cells. Immunoprecipitation experiments indicated that siglec-7 is expressed as a monomer of approximately 65 kDa. |
| spellingShingle | Nicoll, G Ni, J Liu, D Klenerman, P Munday, J Dubock, S Mattei, MG Crocker, P Identification and characterization of a novel siglec, siglec-7, expressed by human natural killer cells and monocytes. |
| title | Identification and characterization of a novel siglec, siglec-7, expressed by human natural killer cells and monocytes. |
| title_full | Identification and characterization of a novel siglec, siglec-7, expressed by human natural killer cells and monocytes. |
| title_fullStr | Identification and characterization of a novel siglec, siglec-7, expressed by human natural killer cells and monocytes. |
| title_full_unstemmed | Identification and characterization of a novel siglec, siglec-7, expressed by human natural killer cells and monocytes. |
| title_short | Identification and characterization of a novel siglec, siglec-7, expressed by human natural killer cells and monocytes. |
| title_sort | identification and characterization of a novel siglec siglec 7 expressed by human natural killer cells and monocytes |
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