A temperature-regulated replicon-based DNA expression system.
We present a temperature-regulated, alphavirus replicon-based DNA expression system. The system is regulated by a viral temperature-sensitive RNA-dependent RNA replicase, creating a temperature-dependent RNA amplification loop. Because of this positive feedback, the system exhibits both low backgrou...
Main Authors: | Boorsma, M, Nieba, L, Koller, D, Bachmann, M, Bailey, J, Renner, W |
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Format: | Journal article |
Language: | English |
Published: |
2000
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