cAMP imaging of cells treated with pertussis toxin, cholera toxin, and anthrax edema toxin.
The enzymatic activity of the three most studied bacterial toxins that increase the cytosolic cAMP level: pertussis toxin (PT), cholera toxin (CT), and anthrax edema toxin (ET), was imaged by fluorescence videomicroscopy. Three different cell lines were transfected with a fluorescence resonance ener...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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| _version_ | 1826282085692735488 |
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| author | Dal Molin, F Zornetta, I Puhar, A Tonello, F Zaccolo, M Montecucco, C |
| author_facet | Dal Molin, F Zornetta, I Puhar, A Tonello, F Zaccolo, M Montecucco, C |
| author_sort | Dal Molin, F |
| collection | OXFORD |
| description | The enzymatic activity of the three most studied bacterial toxins that increase the cytosolic cAMP level: pertussis toxin (PT), cholera toxin (CT), and anthrax edema toxin (ET), was imaged by fluorescence videomicroscopy. Three different cell lines were transfected with a fluorescence resonance energy transfer biosensor based on the PKA regulatory and catalytic subunits fused to CFP and YFP, respectively. Real-time imaging of cells expressing this cAMP biosensor provided time and space resolved pictures of the toxins action. The time course of the PT-induced cAMP increase suggests that its active subunit enters the cytosol more rapidly than that deduced by biochemical experiments. ET generated cAMP concentration gradients decreasing from the nucleus to the cell periphery. On the contrary, CT, which acts on the plasma membrane adenylate cyclase, did not. The potential of imaging methods in studying the mode of entry and the intracellular action of bacterial toxins is discussed. |
| first_indexed | 2024-03-07T00:38:32Z |
| format | Journal article |
| id | oxford-uuid:823e0b0c-948d-465d-9b86-bc69c2ef2716 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:38:32Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:823e0b0c-948d-465d-9b86-bc69c2ef27162022-03-26T21:36:03ZcAMP imaging of cells treated with pertussis toxin, cholera toxin, and anthrax edema toxin.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:823e0b0c-948d-465d-9b86-bc69c2ef2716EnglishSymplectic Elements at Oxford2008Dal Molin, FZornetta, IPuhar, ATonello, FZaccolo, MMontecucco, CThe enzymatic activity of the three most studied bacterial toxins that increase the cytosolic cAMP level: pertussis toxin (PT), cholera toxin (CT), and anthrax edema toxin (ET), was imaged by fluorescence videomicroscopy. Three different cell lines were transfected with a fluorescence resonance energy transfer biosensor based on the PKA regulatory and catalytic subunits fused to CFP and YFP, respectively. Real-time imaging of cells expressing this cAMP biosensor provided time and space resolved pictures of the toxins action. The time course of the PT-induced cAMP increase suggests that its active subunit enters the cytosol more rapidly than that deduced by biochemical experiments. ET generated cAMP concentration gradients decreasing from the nucleus to the cell periphery. On the contrary, CT, which acts on the plasma membrane adenylate cyclase, did not. The potential of imaging methods in studying the mode of entry and the intracellular action of bacterial toxins is discussed. |
| spellingShingle | Dal Molin, F Zornetta, I Puhar, A Tonello, F Zaccolo, M Montecucco, C cAMP imaging of cells treated with pertussis toxin, cholera toxin, and anthrax edema toxin. |
| title | cAMP imaging of cells treated with pertussis toxin, cholera toxin, and anthrax edema toxin. |
| title_full | cAMP imaging of cells treated with pertussis toxin, cholera toxin, and anthrax edema toxin. |
| title_fullStr | cAMP imaging of cells treated with pertussis toxin, cholera toxin, and anthrax edema toxin. |
| title_full_unstemmed | cAMP imaging of cells treated with pertussis toxin, cholera toxin, and anthrax edema toxin. |
| title_short | cAMP imaging of cells treated with pertussis toxin, cholera toxin, and anthrax edema toxin. |
| title_sort | camp imaging of cells treated with pertussis toxin cholera toxin and anthrax edema toxin |
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