Recombinant single-cycle influenza virus with exchangeable pseudotypes allows repeated immunization to augment anti-tumour immunity with immune checkpoint inhibitors
Virus-based tumour vaccines offer many advantages compared to other antigen-delivering systems. They generate concerted innate and adaptive immune response, and robust CD8+ T cell responses. We engineered a non-replicating pseudotyped influenza virus (S-FLU) to deliver the well-known cancer testis a...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
eLife Sciences Publications
2023
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| _version_ | 1797111497115041792 |
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| author | Kandasamy, M Gileadi, U Rijal, P Tan, TK Lee, LN Chen, J Prota, G Klenerman, P Townsend, A Cerundolo, V |
| author_facet | Kandasamy, M Gileadi, U Rijal, P Tan, TK Lee, LN Chen, J Prota, G Klenerman, P Townsend, A Cerundolo, V |
| author_sort | Kandasamy, M |
| collection | OXFORD |
| description | Virus-based tumour vaccines offer many advantages compared to other antigen-delivering systems. They generate concerted innate and adaptive immune response, and robust CD8+ T cell responses. We engineered a non-replicating pseudotyped influenza virus (S-FLU) to deliver the well-known cancer testis antigen, NY-ESO-1 (NY-ESO-1 S-FLU). Intranasal or intramuscular immunization of NY-ESO-1 S-FLU virus in mice elicited a strong NY-ESO-1-specific CD8+ T cell response in lungs and spleen that resulted in the regression of NY-ESO-1-expressing lung tumour and subcutaneous tumour, respectively. Combined administration with anti-PD-1 antibody, NY-ESO-1 S-FLU virus augmented the tumour protection by reducing the tumour metastasis. We propose that the antigen delivery through S-FLU is highly efficient in inducing antigen-specific CD8+ T cell response and protection against tumour development in combination with PD-1 blockade. |
| first_indexed | 2024-03-07T08:09:45Z |
| format | Journal article |
| id | oxford-uuid:82b786bf-25a4-4b65-bcc4-dd227238eeb5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:09:45Z |
| publishDate | 2023 |
| publisher | eLife Sciences Publications |
| record_format | dspace |
| spelling | oxford-uuid:82b786bf-25a4-4b65-bcc4-dd227238eeb52023-11-20T17:18:52ZRecombinant single-cycle influenza virus with exchangeable pseudotypes allows repeated immunization to augment anti-tumour immunity with immune checkpoint inhibitorsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:82b786bf-25a4-4b65-bcc4-dd227238eeb5EnglishSymplectic ElementseLife Sciences Publications2023Kandasamy, MGileadi, URijal, PTan, TKLee, LNChen, JProta, GKlenerman, PTownsend, ACerundolo, VVirus-based tumour vaccines offer many advantages compared to other antigen-delivering systems. They generate concerted innate and adaptive immune response, and robust CD8+ T cell responses. We engineered a non-replicating pseudotyped influenza virus (S-FLU) to deliver the well-known cancer testis antigen, NY-ESO-1 (NY-ESO-1 S-FLU). Intranasal or intramuscular immunization of NY-ESO-1 S-FLU virus in mice elicited a strong NY-ESO-1-specific CD8+ T cell response in lungs and spleen that resulted in the regression of NY-ESO-1-expressing lung tumour and subcutaneous tumour, respectively. Combined administration with anti-PD-1 antibody, NY-ESO-1 S-FLU virus augmented the tumour protection by reducing the tumour metastasis. We propose that the antigen delivery through S-FLU is highly efficient in inducing antigen-specific CD8+ T cell response and protection against tumour development in combination with PD-1 blockade. |
| spellingShingle | Kandasamy, M Gileadi, U Rijal, P Tan, TK Lee, LN Chen, J Prota, G Klenerman, P Townsend, A Cerundolo, V Recombinant single-cycle influenza virus with exchangeable pseudotypes allows repeated immunization to augment anti-tumour immunity with immune checkpoint inhibitors |
| title | Recombinant single-cycle influenza virus with exchangeable pseudotypes allows repeated immunization to augment anti-tumour immunity with immune checkpoint inhibitors |
| title_full | Recombinant single-cycle influenza virus with exchangeable pseudotypes allows repeated immunization to augment anti-tumour immunity with immune checkpoint inhibitors |
| title_fullStr | Recombinant single-cycle influenza virus with exchangeable pseudotypes allows repeated immunization to augment anti-tumour immunity with immune checkpoint inhibitors |
| title_full_unstemmed | Recombinant single-cycle influenza virus with exchangeable pseudotypes allows repeated immunization to augment anti-tumour immunity with immune checkpoint inhibitors |
| title_short | Recombinant single-cycle influenza virus with exchangeable pseudotypes allows repeated immunization to augment anti-tumour immunity with immune checkpoint inhibitors |
| title_sort | recombinant single cycle influenza virus with exchangeable pseudotypes allows repeated immunization to augment anti tumour immunity with immune checkpoint inhibitors |
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