Rev binds specifically to a purine loop in the SL1 region of the HIV-1 leader RNA.

Rev binds specifically to a purine loop in the SL1 region of the HIV-1 leader RNA.

The leader RNA sequence of human immunodeficiency virus type 1 (HIV-1) consists of a complex series of stem loop structures that are critical for viral replication. Three-dimensional structural analysis by NMR of one of these structures, the SL1 stem loop of the packaging signal region, revealed a h...

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Մատենագիտական մանրամասներ
Հիմնական հեղինակներ: Gallego, J, Greatorex, J, Zhang, H, Yang, B, Arunachalam, S, Fang, J, Seamons, J, Lea, S, Pomerantz, R, Lever, A
Ձևաչափ: Journal article
Լեզու:English
Հրապարակվել է: 2003
Նկարագրություն
Ամփոփում:The leader RNA sequence of human immunodeficiency virus type 1 (HIV-1) consists of a complex series of stem loop structures that are critical for viral replication. Three-dimensional structural analysis by NMR of one of these structures, the SL1 stem loop of the packaging signal region, revealed a highly conserved purine rich loop with a structure nearly identical to the Rev-binding loop of the Rev response element. Using band-shift assays, surface plasmon resonance, and further NMR analysis, we demonstrate that this loop binds Rev. HIV-1 appears to have a second Rev-binding site close to the major splice donor site that may have an additional role in the viral life cycle.

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