Rev binds specifically to a purine loop in the SL1 region of the HIV-1 leader RNA.
The leader RNA sequence of human immunodeficiency virus type 1 (HIV-1) consists of a complex series of stem loop structures that are critical for viral replication. Three-dimensional structural analysis by NMR of one of these structures, the SL1 stem loop of the packaging signal region, revealed a h...
| Autori principali: | , , , , , , , , , |
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| Natura: | Journal article |
| Lingua: | English |
| Pubblicazione: |
2003
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| _version_ | 1826282185788751872 |
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| author | Gallego, J Greatorex, J Zhang, H Yang, B Arunachalam, S Fang, J Seamons, J Lea, S Pomerantz, R Lever, A |
| author_facet | Gallego, J Greatorex, J Zhang, H Yang, B Arunachalam, S Fang, J Seamons, J Lea, S Pomerantz, R Lever, A |
| author_sort | Gallego, J |
| collection | OXFORD |
| description | The leader RNA sequence of human immunodeficiency virus type 1 (HIV-1) consists of a complex series of stem loop structures that are critical for viral replication. Three-dimensional structural analysis by NMR of one of these structures, the SL1 stem loop of the packaging signal region, revealed a highly conserved purine rich loop with a structure nearly identical to the Rev-binding loop of the Rev response element. Using band-shift assays, surface plasmon resonance, and further NMR analysis, we demonstrate that this loop binds Rev. HIV-1 appears to have a second Rev-binding site close to the major splice donor site that may have an additional role in the viral life cycle. |
| first_indexed | 2024-03-07T00:40:01Z |
| format | Journal article |
| id | oxford-uuid:82ba6d8c-c6b1-494a-a8a1-e14f9aa0c0c4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:40:01Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:82ba6d8c-c6b1-494a-a8a1-e14f9aa0c0c42022-03-26T21:39:21ZRev binds specifically to a purine loop in the SL1 region of the HIV-1 leader RNA.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:82ba6d8c-c6b1-494a-a8a1-e14f9aa0c0c4EnglishSymplectic Elements at Oxford2003Gallego, JGreatorex, JZhang, HYang, BArunachalam, SFang, JSeamons, JLea, SPomerantz, RLever, AThe leader RNA sequence of human immunodeficiency virus type 1 (HIV-1) consists of a complex series of stem loop structures that are critical for viral replication. Three-dimensional structural analysis by NMR of one of these structures, the SL1 stem loop of the packaging signal region, revealed a highly conserved purine rich loop with a structure nearly identical to the Rev-binding loop of the Rev response element. Using band-shift assays, surface plasmon resonance, and further NMR analysis, we demonstrate that this loop binds Rev. HIV-1 appears to have a second Rev-binding site close to the major splice donor site that may have an additional role in the viral life cycle. |
| spellingShingle | Gallego, J Greatorex, J Zhang, H Yang, B Arunachalam, S Fang, J Seamons, J Lea, S Pomerantz, R Lever, A Rev binds specifically to a purine loop in the SL1 region of the HIV-1 leader RNA. |
| title | Rev binds specifically to a purine loop in the SL1 region of the HIV-1 leader RNA. |
| title_full | Rev binds specifically to a purine loop in the SL1 region of the HIV-1 leader RNA. |
| title_fullStr | Rev binds specifically to a purine loop in the SL1 region of the HIV-1 leader RNA. |
| title_full_unstemmed | Rev binds specifically to a purine loop in the SL1 region of the HIV-1 leader RNA. |
| title_short | Rev binds specifically to a purine loop in the SL1 region of the HIV-1 leader RNA. |
| title_sort | rev binds specifically to a purine loop in the sl1 region of the hiv 1 leader rna |
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