Cell-mediated immune response in vitro. II. The role of thymus and thymus-derived lymphocytes.
A recently developed allograft system for the generation of a cell-mediated response in tissue culture was applied to an analysis of the cells involved in that response. Lymphoid cells from CBA mice (H-2k) were immunized in vitro with mitomycin-treated BALB/c (H-2d) spleen cells and the cell-mediate...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1972
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| _version_ | 1797079061377318912 |
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| author | Wagner, H Harris, A Feldmann, M |
| author_facet | Wagner, H Harris, A Feldmann, M |
| author_sort | Wagner, H |
| collection | OXFORD |
| description | A recently developed allograft system for the generation of a cell-mediated response in tissue culture was applied to an analysis of the cells involved in that response. Lymphoid cells from CBA mice (H-2k) were immunized in vitro with mitomycin-treated BALB/c (H-2d) spleen cells and the cell-mediated response measured by lysis of 51Cr-labeled P815 mastocytoma cells (H-2d). It was found that in mouse spleen the precursor of cytotoxic lymphocytes, as well as the effector cells themselves are θ positive lymphocytes, a characteristic of T cells. Normal mouse thymocytes alone differentiate in vitro into cytotoxic effector cells, thus excluding a thymus bone marrow synergism in the allograft response in vitro. Various lymphoid cell populations were assayed as sources of precursor cells for the cell-mediated response. Purified splenic T lymphocytes were as active as cortisone-resistant thymocytes which were 10- 12-fold more active than thymus cells. Spleen lymphocytes were 3-fold more active. In vitro immunization of cortisone-resistant thymocytes resulted in the most active killer cell population. Fifty percent of the target cells were lysed within 150 min at a lymphocyte-to-target cell ratio of 1 to 1 or less. Such activity is higher than any previously reported from in vivo immunization procedures. © 1972. |
| first_indexed | 2024-03-07T00:40:17Z |
| format | Journal article |
| id | oxford-uuid:82ce3db9-4f99-4484-9c67-a78175851255 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:40:17Z |
| publishDate | 1972 |
| record_format | dspace |
| spelling | oxford-uuid:82ce3db9-4f99-4484-9c67-a781758512552022-03-26T21:39:53ZCell-mediated immune response in vitro. II. The role of thymus and thymus-derived lymphocytes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:82ce3db9-4f99-4484-9c67-a78175851255EnglishSymplectic Elements at Oxford1972Wagner, HHarris, AFeldmann, MA recently developed allograft system for the generation of a cell-mediated response in tissue culture was applied to an analysis of the cells involved in that response. Lymphoid cells from CBA mice (H-2k) were immunized in vitro with mitomycin-treated BALB/c (H-2d) spleen cells and the cell-mediated response measured by lysis of 51Cr-labeled P815 mastocytoma cells (H-2d). It was found that in mouse spleen the precursor of cytotoxic lymphocytes, as well as the effector cells themselves are θ positive lymphocytes, a characteristic of T cells. Normal mouse thymocytes alone differentiate in vitro into cytotoxic effector cells, thus excluding a thymus bone marrow synergism in the allograft response in vitro. Various lymphoid cell populations were assayed as sources of precursor cells for the cell-mediated response. Purified splenic T lymphocytes were as active as cortisone-resistant thymocytes which were 10- 12-fold more active than thymus cells. Spleen lymphocytes were 3-fold more active. In vitro immunization of cortisone-resistant thymocytes resulted in the most active killer cell population. Fifty percent of the target cells were lysed within 150 min at a lymphocyte-to-target cell ratio of 1 to 1 or less. Such activity is higher than any previously reported from in vivo immunization procedures. © 1972. |
| spellingShingle | Wagner, H Harris, A Feldmann, M Cell-mediated immune response in vitro. II. The role of thymus and thymus-derived lymphocytes. |
| title | Cell-mediated immune response in vitro. II. The role of thymus and thymus-derived lymphocytes. |
| title_full | Cell-mediated immune response in vitro. II. The role of thymus and thymus-derived lymphocytes. |
| title_fullStr | Cell-mediated immune response in vitro. II. The role of thymus and thymus-derived lymphocytes. |
| title_full_unstemmed | Cell-mediated immune response in vitro. II. The role of thymus and thymus-derived lymphocytes. |
| title_short | Cell-mediated immune response in vitro. II. The role of thymus and thymus-derived lymphocytes. |
| title_sort | cell mediated immune response in vitro ii the role of thymus and thymus derived lymphocytes |
| work_keys_str_mv | AT wagnerh cellmediatedimmuneresponseinvitroiitheroleofthymusandthymusderivedlymphocytes AT harrisa cellmediatedimmuneresponseinvitroiitheroleofthymusandthymusderivedlymphocytes AT feldmannm cellmediatedimmuneresponseinvitroiitheroleofthymusandthymusderivedlymphocytes |