T cell receptor usage of virus-specific CD8 cells and recognition of viral mutations during acute and persistent hepatitis B virus infection.
T cells specific for a single viral epitope, but using different T cell receptors, should have flexibility in their epitope recognition to protect the infected host against the emergence of viral escape mutants. Therefore, polyclonality of the hepatitis B virus (HBV)-specific cytotoxic T lymphocyte...
| Autori principali: | , , , , , , , |
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| Natura: | Journal article |
| Lingua: | English |
| Pubblicazione: |
2000
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| _version_ | 1826282239880593408 |
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| author | Maini, M Reignat, S Boni, C Ogg, G King, A Malacarne, F Webster, G Bertoletti, A |
| author_facet | Maini, M Reignat, S Boni, C Ogg, G King, A Malacarne, F Webster, G Bertoletti, A |
| author_sort | Maini, M |
| collection | OXFORD |
| description | T cells specific for a single viral epitope, but using different T cell receptors, should have flexibility in their epitope recognition to protect the infected host against the emergence of viral escape mutants. Therefore, polyclonality of the hepatitis B virus (HBV)-specific cytotoxic T lymphocyte response has been hypothesized to be a major determinant in the control of infection. We analyzed the Vbeta chain composition of the core 18-27-specific CD8 cells in acute and persistently HBV-infected patients using HLA-A2 tetrameric complexes and a panel of Vbeta antibodies. Different T cell receptors were utilized by core 18-27-specific CD8 cells both in patients with acute and chronic infection. The functional ability of these epitope-specific T cells to respond to potential viral mutations was then tested. The polyclonal HBV-specific CD8 response present in patients with acute hepatitis displayed a limited efficiency to recognize mutations introduced within the epitope. The ability of core 18-27-specific CD8 to tolerate epitope mutations was found only during persistent HBV infection. The data suggest that although a clonally heterogeneous CD8 response can be largely inhibited by the occurrence of single epitope mutations in primary HBV infection, preferential selection of T cells able to counteract the emergence of viral mutations can occur during persistent infection. |
| first_indexed | 2024-03-07T00:40:51Z |
| format | Journal article |
| id | oxford-uuid:82fc6d85-70e8-419a-ba4b-13ea9dd83d0f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:40:51Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:82fc6d85-70e8-419a-ba4b-13ea9dd83d0f2022-03-26T21:41:11ZT cell receptor usage of virus-specific CD8 cells and recognition of viral mutations during acute and persistent hepatitis B virus infection.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:82fc6d85-70e8-419a-ba4b-13ea9dd83d0fEnglishSymplectic Elements at Oxford2000Maini, MReignat, SBoni, COgg, GKing, AMalacarne, FWebster, GBertoletti, AT cells specific for a single viral epitope, but using different T cell receptors, should have flexibility in their epitope recognition to protect the infected host against the emergence of viral escape mutants. Therefore, polyclonality of the hepatitis B virus (HBV)-specific cytotoxic T lymphocyte response has been hypothesized to be a major determinant in the control of infection. We analyzed the Vbeta chain composition of the core 18-27-specific CD8 cells in acute and persistently HBV-infected patients using HLA-A2 tetrameric complexes and a panel of Vbeta antibodies. Different T cell receptors were utilized by core 18-27-specific CD8 cells both in patients with acute and chronic infection. The functional ability of these epitope-specific T cells to respond to potential viral mutations was then tested. The polyclonal HBV-specific CD8 response present in patients with acute hepatitis displayed a limited efficiency to recognize mutations introduced within the epitope. The ability of core 18-27-specific CD8 to tolerate epitope mutations was found only during persistent HBV infection. The data suggest that although a clonally heterogeneous CD8 response can be largely inhibited by the occurrence of single epitope mutations in primary HBV infection, preferential selection of T cells able to counteract the emergence of viral mutations can occur during persistent infection. |
| spellingShingle | Maini, M Reignat, S Boni, C Ogg, G King, A Malacarne, F Webster, G Bertoletti, A T cell receptor usage of virus-specific CD8 cells and recognition of viral mutations during acute and persistent hepatitis B virus infection. |
| title | T cell receptor usage of virus-specific CD8 cells and recognition of viral mutations during acute and persistent hepatitis B virus infection. |
| title_full | T cell receptor usage of virus-specific CD8 cells and recognition of viral mutations during acute and persistent hepatitis B virus infection. |
| title_fullStr | T cell receptor usage of virus-specific CD8 cells and recognition of viral mutations during acute and persistent hepatitis B virus infection. |
| title_full_unstemmed | T cell receptor usage of virus-specific CD8 cells and recognition of viral mutations during acute and persistent hepatitis B virus infection. |
| title_short | T cell receptor usage of virus-specific CD8 cells and recognition of viral mutations during acute and persistent hepatitis B virus infection. |
| title_sort | t cell receptor usage of virus specific cd8 cells and recognition of viral mutations during acute and persistent hepatitis b virus infection |
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