Intergenic transcription and transinduction of the human beta-globin locus.
We have identified novel nuclear transcripts in the human beta-globin locus using nuclear run-on analysis in erythroid cell lines and in situ hybridization analysis of erythroid tissue. These transcripts extend across the LCR and intergenic regions but are undetectable in nonerythroid cells. Surpris...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1997
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| _version_ | 1797079102659756032 |
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| author | Ashe, H Monks, J Wijgerde, M Fraser, P Proudfoot, N |
| author_facet | Ashe, H Monks, J Wijgerde, M Fraser, P Proudfoot, N |
| author_sort | Ashe, H |
| collection | OXFORD |
| description | We have identified novel nuclear transcripts in the human beta-globin locus using nuclear run-on analysis in erythroid cell lines and in situ hybridization analysis of erythroid tissue. These transcripts extend across the LCR and intergenic regions but are undetectable in nonerythroid cells. Surprisingly, transient transfection of a beta-globin gene (epsilon, gamma, or beta) induces transcription of the LCR and intergenic regions from the chromosomal beta-globin locus in nonerythroid cell lines. The beta-globin genes themselves, however, remain transcriptionally silent. Induction is dependent on transcription of the globin gene in the transfected plasmid but does not require protein expression. Using in situ hybridization analysis, we show that the plasmid colocalizes with the endogenous beta-globin locus providing insight into the mechanism of transinduction. |
| first_indexed | 2024-03-07T00:40:54Z |
| format | Journal article |
| id | oxford-uuid:83011577-a3df-451e-8176-362161ba6b30 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:40:54Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:83011577-a3df-451e-8176-362161ba6b302022-03-26T21:41:20ZIntergenic transcription and transinduction of the human beta-globin locus.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:83011577-a3df-451e-8176-362161ba6b30EnglishSymplectic Elements at Oxford1997Ashe, HMonks, JWijgerde, MFraser, PProudfoot, NWe have identified novel nuclear transcripts in the human beta-globin locus using nuclear run-on analysis in erythroid cell lines and in situ hybridization analysis of erythroid tissue. These transcripts extend across the LCR and intergenic regions but are undetectable in nonerythroid cells. Surprisingly, transient transfection of a beta-globin gene (epsilon, gamma, or beta) induces transcription of the LCR and intergenic regions from the chromosomal beta-globin locus in nonerythroid cell lines. The beta-globin genes themselves, however, remain transcriptionally silent. Induction is dependent on transcription of the globin gene in the transfected plasmid but does not require protein expression. Using in situ hybridization analysis, we show that the plasmid colocalizes with the endogenous beta-globin locus providing insight into the mechanism of transinduction. |
| spellingShingle | Ashe, H Monks, J Wijgerde, M Fraser, P Proudfoot, N Intergenic transcription and transinduction of the human beta-globin locus. |
| title | Intergenic transcription and transinduction of the human beta-globin locus. |
| title_full | Intergenic transcription and transinduction of the human beta-globin locus. |
| title_fullStr | Intergenic transcription and transinduction of the human beta-globin locus. |
| title_full_unstemmed | Intergenic transcription and transinduction of the human beta-globin locus. |
| title_short | Intergenic transcription and transinduction of the human beta-globin locus. |
| title_sort | intergenic transcription and transinduction of the human beta globin locus |
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