A novel human artificial chromosome gene expression system using herpes simplex virus type 1 vectors.
Human artificial chromosome (HAC) vectors are an important gene transfer system for expression and complementation studies. We describe a significant advance in HAC technology using infectious herpes simplex virus type 1 (HSV-1) amplicon vectors for delivery. This highly efficient method has allowed...
| Main Authors: | , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2006
|
| _version_ | 1826282313720266752 |
|---|---|
| author | Moralli, D Simpson, K Wade-Martins, R Monaco, Z |
| author_facet | Moralli, D Simpson, K Wade-Martins, R Monaco, Z |
| author_sort | Moralli, D |
| collection | OXFORD |
| description | Human artificial chromosome (HAC) vectors are an important gene transfer system for expression and complementation studies. We describe a significant advance in HAC technology using infectious herpes simplex virus type 1 (HSV-1) amplicon vectors for delivery. This highly efficient method has allowed gene-expressing HACs to be established in glioma-, kidney- and lung-derived cells. We also developed an HSV-1 hypoxanthine phosphoribosyltransferase (HPRT) HAC vector, which generated functional HPRT-expressing HACs that complemented the genetic deficiency in human cells. The transduction efficiency of the HSV-1 HAC amplicons is several orders of magnitude higher than lipofection-mediated delivery. Studies on HAC stability between cell types showed important differences that have implications for HAC development and gene expression in human cells. This is the first report of establishing gene-expressing HACs in human cells by using an efficient, high-capacity viral vector and by identifying factors that are involved in cell-type-specific HAC instability. The work is a significant advance for HAC technology and the development of HAC gene expression systems in human cells. |
| first_indexed | 2024-03-07T00:41:58Z |
| format | Journal article |
| id | oxford-uuid:8359cd81-66eb-43a6-b77b-9e13e0183a66 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:41:58Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:8359cd81-66eb-43a6-b77b-9e13e0183a662022-03-26T21:43:37ZA novel human artificial chromosome gene expression system using herpes simplex virus type 1 vectors.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8359cd81-66eb-43a6-b77b-9e13e0183a66EnglishSymplectic Elements at Oxford2006Moralli, DSimpson, KWade-Martins, RMonaco, ZHuman artificial chromosome (HAC) vectors are an important gene transfer system for expression and complementation studies. We describe a significant advance in HAC technology using infectious herpes simplex virus type 1 (HSV-1) amplicon vectors for delivery. This highly efficient method has allowed gene-expressing HACs to be established in glioma-, kidney- and lung-derived cells. We also developed an HSV-1 hypoxanthine phosphoribosyltransferase (HPRT) HAC vector, which generated functional HPRT-expressing HACs that complemented the genetic deficiency in human cells. The transduction efficiency of the HSV-1 HAC amplicons is several orders of magnitude higher than lipofection-mediated delivery. Studies on HAC stability between cell types showed important differences that have implications for HAC development and gene expression in human cells. This is the first report of establishing gene-expressing HACs in human cells by using an efficient, high-capacity viral vector and by identifying factors that are involved in cell-type-specific HAC instability. The work is a significant advance for HAC technology and the development of HAC gene expression systems in human cells. |
| spellingShingle | Moralli, D Simpson, K Wade-Martins, R Monaco, Z A novel human artificial chromosome gene expression system using herpes simplex virus type 1 vectors. |
| title | A novel human artificial chromosome gene expression system using herpes simplex virus type 1 vectors. |
| title_full | A novel human artificial chromosome gene expression system using herpes simplex virus type 1 vectors. |
| title_fullStr | A novel human artificial chromosome gene expression system using herpes simplex virus type 1 vectors. |
| title_full_unstemmed | A novel human artificial chromosome gene expression system using herpes simplex virus type 1 vectors. |
| title_short | A novel human artificial chromosome gene expression system using herpes simplex virus type 1 vectors. |
| title_sort | novel human artificial chromosome gene expression system using herpes simplex virus type 1 vectors |
| work_keys_str_mv | AT morallid anovelhumanartificialchromosomegeneexpressionsystemusingherpessimplexvirustype1vectors AT simpsonk anovelhumanartificialchromosomegeneexpressionsystemusingherpessimplexvirustype1vectors AT wademartinsr anovelhumanartificialchromosomegeneexpressionsystemusingherpessimplexvirustype1vectors AT monacoz anovelhumanartificialchromosomegeneexpressionsystemusingherpessimplexvirustype1vectors AT morallid novelhumanartificialchromosomegeneexpressionsystemusingherpessimplexvirustype1vectors AT simpsonk novelhumanartificialchromosomegeneexpressionsystemusingherpessimplexvirustype1vectors AT wademartinsr novelhumanartificialchromosomegeneexpressionsystemusingherpessimplexvirustype1vectors AT monacoz novelhumanartificialchromosomegeneexpressionsystemusingherpessimplexvirustype1vectors |