Aberrations and adaptive optics in super-resolution microscopy
As one of the most powerful tools in the biological investigation of cellular structures and dynamic processes, fluorescence microscopy has undergone extraordinary developments in the past decades. The advent of super-resolution techniques has enabled fluorescence microscopy – or rather nanoscopy –...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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Oxford University Press
2015
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| _version_ | 1797079321401098240 |
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| author | Booth, M Andrade, D Burke, D Patton, B Zurauskas, M |
| author_facet | Booth, M Andrade, D Burke, D Patton, B Zurauskas, M |
| author_sort | Booth, M |
| collection | OXFORD |
| description | As one of the most powerful tools in the biological investigation of cellular structures and dynamic processes, fluorescence microscopy has undergone extraordinary developments in the past decades. The advent of super-resolution techniques has enabled fluorescence microscopy – or rather nanoscopy – to achieve nanoscale resolution in living specimens and unravelled the interior of cells with unprecedented detail. The methods employed in this expanding field of microscopy, however, are especially prone to the detrimental effects of optical aberrations. In this review, we discuss how super-resolution microscopy techniques based upon single-molecule switching, stimulated emission depletion and structured illumination each suffer from aberrations in different ways that are dependent upon intrinsic technical aspects. We discuss the use of adaptive optics as an effective means to overcome this problem. |
| first_indexed | 2024-03-07T00:44:10Z |
| format | Journal article |
| id | oxford-uuid:840d021d-9eae-4414-a58e-ada92f2f3490 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:44:10Z |
| publishDate | 2015 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:840d021d-9eae-4414-a58e-ada92f2f34902022-03-26T21:48:30ZAberrations and adaptive optics in super-resolution microscopyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:840d021d-9eae-4414-a58e-ada92f2f3490EnglishSymplectic Elements at OxfordOxford University Press2015Booth, MAndrade, DBurke, DPatton, BZurauskas, MAs one of the most powerful tools in the biological investigation of cellular structures and dynamic processes, fluorescence microscopy has undergone extraordinary developments in the past decades. The advent of super-resolution techniques has enabled fluorescence microscopy – or rather nanoscopy – to achieve nanoscale resolution in living specimens and unravelled the interior of cells with unprecedented detail. The methods employed in this expanding field of microscopy, however, are especially prone to the detrimental effects of optical aberrations. In this review, we discuss how super-resolution microscopy techniques based upon single-molecule switching, stimulated emission depletion and structured illumination each suffer from aberrations in different ways that are dependent upon intrinsic technical aspects. We discuss the use of adaptive optics as an effective means to overcome this problem. |
| spellingShingle | Booth, M Andrade, D Burke, D Patton, B Zurauskas, M Aberrations and adaptive optics in super-resolution microscopy |
| title | Aberrations and adaptive optics in super-resolution microscopy |
| title_full | Aberrations and adaptive optics in super-resolution microscopy |
| title_fullStr | Aberrations and adaptive optics in super-resolution microscopy |
| title_full_unstemmed | Aberrations and adaptive optics in super-resolution microscopy |
| title_short | Aberrations and adaptive optics in super-resolution microscopy |
| title_sort | aberrations and adaptive optics in super resolution microscopy |
| work_keys_str_mv | AT boothm aberrationsandadaptiveopticsinsuperresolutionmicroscopy AT andraded aberrationsandadaptiveopticsinsuperresolutionmicroscopy AT burked aberrationsandadaptiveopticsinsuperresolutionmicroscopy AT pattonb aberrationsandadaptiveopticsinsuperresolutionmicroscopy AT zurauskasm aberrationsandadaptiveopticsinsuperresolutionmicroscopy |