TNF autovaccination induces self anti-TNF antibodies and inhibits metastasis in a murine melanoma model.
TNF is a proinflammatory cytokine involved in the pathogenesis of chronic inflammatory diseases, but also in metastasis in certain types of cancer. In terms of therapy, TNF is targeted by anti-TNF neutralising monoclonal antibodies or soluble TNF receptors. Recently, a novel strategy based on the ge...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Nature Publishing Group
2004
|
| _version_ | 1826282465540440064 |
|---|---|
| author | Waterston, A Salway, F Andreakos, E Butler, D Feldmann, M Coombes, R |
| author_facet | Waterston, A Salway, F Andreakos, E Butler, D Feldmann, M Coombes, R |
| author_sort | Waterston, A |
| collection | OXFORD |
| description | TNF is a proinflammatory cytokine involved in the pathogenesis of chronic inflammatory diseases, but also in metastasis in certain types of cancer. In terms of therapy, TNF is targeted by anti-TNF neutralising monoclonal antibodies or soluble TNF receptors. Recently, a novel strategy based on the generation of self anti-TNF antibodies (TNF autovaccination) has been developed. We have previously shown that TNF autovaccination successfully generates high anti-TNF antibody titres, blocks TNF and ameliorates collagen-induced arthritis in DBA/1 mice. In this study, we examined the ability of TNF autovaccination to generate anti-TNF antibody titres and block metastasis in the murine B16F10 melanoma model. We found that immunisation of C57BL/6 mice with TNF autovaccine produces a 100-fold antibody response to TNF compared to immunisation with phosphate-buffered saline vehicle control and significantly reduces both the number (P<0.01) and size of metastases (P<0.01) of B16F10 melanoma cells. This effect is also observed when an anti-TNF neutralising monoclonal antibody is administered, confirming the essential role TNF plays in metastasis in this model. This study suggests that TNF autovaccination is a cheaper and highly efficient alternative that can block TNF and reduce metastasis in vivo and trials with TNF autovaccination are already underway in patients with metastatic cancer. |
| first_indexed | 2024-03-07T00:44:15Z |
| format | Journal article |
| id | oxford-uuid:84145208-c67e-4e13-b572-f37015164e6c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:44:15Z |
| publishDate | 2004 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | oxford-uuid:84145208-c67e-4e13-b572-f37015164e6c2022-03-26T21:48:44ZTNF autovaccination induces self anti-TNF antibodies and inhibits metastasis in a murine melanoma model.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:84145208-c67e-4e13-b572-f37015164e6cEnglishSymplectic Elements at OxfordNature Publishing Group2004Waterston, ASalway, FAndreakos, EButler, DFeldmann, MCoombes, RTNF is a proinflammatory cytokine involved in the pathogenesis of chronic inflammatory diseases, but also in metastasis in certain types of cancer. In terms of therapy, TNF is targeted by anti-TNF neutralising monoclonal antibodies or soluble TNF receptors. Recently, a novel strategy based on the generation of self anti-TNF antibodies (TNF autovaccination) has been developed. We have previously shown that TNF autovaccination successfully generates high anti-TNF antibody titres, blocks TNF and ameliorates collagen-induced arthritis in DBA/1 mice. In this study, we examined the ability of TNF autovaccination to generate anti-TNF antibody titres and block metastasis in the murine B16F10 melanoma model. We found that immunisation of C57BL/6 mice with TNF autovaccine produces a 100-fold antibody response to TNF compared to immunisation with phosphate-buffered saline vehicle control and significantly reduces both the number (P<0.01) and size of metastases (P<0.01) of B16F10 melanoma cells. This effect is also observed when an anti-TNF neutralising monoclonal antibody is administered, confirming the essential role TNF plays in metastasis in this model. This study suggests that TNF autovaccination is a cheaper and highly efficient alternative that can block TNF and reduce metastasis in vivo and trials with TNF autovaccination are already underway in patients with metastatic cancer. |
| spellingShingle | Waterston, A Salway, F Andreakos, E Butler, D Feldmann, M Coombes, R TNF autovaccination induces self anti-TNF antibodies and inhibits metastasis in a murine melanoma model. |
| title | TNF autovaccination induces self anti-TNF antibodies and inhibits metastasis in a murine melanoma model. |
| title_full | TNF autovaccination induces self anti-TNF antibodies and inhibits metastasis in a murine melanoma model. |
| title_fullStr | TNF autovaccination induces self anti-TNF antibodies and inhibits metastasis in a murine melanoma model. |
| title_full_unstemmed | TNF autovaccination induces self anti-TNF antibodies and inhibits metastasis in a murine melanoma model. |
| title_short | TNF autovaccination induces self anti-TNF antibodies and inhibits metastasis in a murine melanoma model. |
| title_sort | tnf autovaccination induces self anti tnf antibodies and inhibits metastasis in a murine melanoma model |
| work_keys_str_mv | AT waterstona tnfautovaccinationinducesselfantitnfantibodiesandinhibitsmetastasisinamurinemelanomamodel AT salwayf tnfautovaccinationinducesselfantitnfantibodiesandinhibitsmetastasisinamurinemelanomamodel AT andreakose tnfautovaccinationinducesselfantitnfantibodiesandinhibitsmetastasisinamurinemelanomamodel AT butlerd tnfautovaccinationinducesselfantitnfantibodiesandinhibitsmetastasisinamurinemelanomamodel AT feldmannm tnfautovaccinationinducesselfantitnfantibodiesandinhibitsmetastasisinamurinemelanomamodel AT coombesr tnfautovaccinationinducesselfantitnfantibodiesandinhibitsmetastasisinamurinemelanomamodel |